2026-06-29T08:13:43Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/949952024-07-18T00:04:04Zcom_20.500.14352_14col_20.500.14352_15

Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2 Ruiz Valdepeñas Montiel, Víctor Pellicanò, Alessandro Campuzano Ruiz, Susana Torrente Rodríguez, Rebeca Magnolia Reviejo García, Ángel Julio Cosio, Maria Stella Pingarrón Carrazón, José Manuel 543 Ara h 2 Magnetic beads Screen-printed electrode Amperometric immunosensor Food extracts Química 2301 Química Analítica A highly sensitive disposable amperometric magnetoimmunosensor for the rapid determination of Ara h 2 protein, one of the major peanut allergens, is reported. The approach uses a sandwich configuration involving selective capture and detector antibodies and carboxylic acid-modified magnetic beads (HOOC-MBs). Detector antibodies are labeled with HRP-conjugated secondary antibodies and the MBs bearing the immunoconjugates are magnetically captured on surface of a disposable screen-printed carbon electrode (SPCE). The affinity reactions are monitored amperometrically at −0.20 V (vs a Ag pseudo-reference electrode) in the presence of hydroquinone (HQ) as electron transfer mediator and upon addition of hydrogen peroxide as the enzyme substrate. The developed magnetoimmunosensor exhibits a wide range of linearity between 87 and 10,000 pg/mL Ara h 2 with a detection limit of 26 pg/mL as well as a great selectivity against other non-target proteins. The magnetoimmunosensing platform was successfully applied for the detection of Ara h 2 in different food extracts. After an appropriate sample dilution no matrix effects were observable. The developed methodology was able to detect trace amounts of the peanut allergen (0.0005% or 5.0 mg/kg) in wheat flour spiked samples. The results correlated properly with those provided by a commercial ELISA kit. Ministerio de Economía y Competitividad (España) Comunidad de Madrid Ministry of Education (Italy) Depto. de Química Analítica Fac. de Ciencias Químicas TRUE pub 2024-01-24T09:44:37Z 2024-01-24T09:44:37Z 2016 journal article VoR https://hdl.handle.net/20.500.14352/94995 0925-4005 10.1016/j.snb.2016.01.123 eng info:eu-repo/grantAgreement/MINECO//CTQ2015-64402-C2-1-R/ES/NUEVAS PLATAFORMAS DE MULTIPLEXADO ELECTROANALITICAS PARA LA DETECCION Y PRONOSTICO DE ENFERMEDADES NEOPLASICAS MEDIANTE BIOPSIAS LIQUIDAS/ S2013/MT-3029 MIUR, 2010 AXENJ8_002 Montiel, V. R. V., Pellicano, A., Campuzano, S., Torrente-Rodríguez, R. M., Reviejo, Á. J., Cosio, M. S., & Pingarrón, J. M. (2016). Electrochemical detection of peanuts at trace levels in foods using a magnetoimmunosensor for the allergenic protein Ara h 2. Sensors and Actuators B: Chemical, 236, 825-833. restricted access application/pdf Elsevier Science