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oai:docta.ucm.es:20.500.14352/951482025-07-10T00:11:14Zcom_20.500.14352_14col_20.500.14352_15

Sánchez-Aguilera López, Alberto Sánchez-Alonso, José Luis Vicente Torres, María Ángeles Colino Matilla, María Asunción 2024-01-24T14:05:22Z 2024-01-24T14:05:22Z 2016 Sánchez-Aguilera, Alberto, et al. «Role of Low-Voltage-Activated Calcium Current and Extracellular Calcium in Controlling the Firing Pattern of Developing CA1 Pyramidal Neurons». Neuroscience, vol. 344, marzo de 2017, pp. 89-101. https://doi.org/10.1016/j.neuroscience.2016.12.024. 0306-4522 10.1016/j.neuroscience.2016.12.024 https://hdl.handle.net/20.500.14352/95148 1873-7544 https://doi.org/10.1016/j.neuroscience.2016.12.024 28039042 The firing pattern of individual neurons is an important element for information processing and storing. During the first weeks of development, there is a transitional period during which CA1 pyramidal neurons display burst-spiking behavior in contrast to the adult regular-firing pattern. Spike after-depolarizations (ADPs) constitute a major factor underlying burst-spiking behavior. Using current-clamp recordings, we studied ADP waveforms and firing patterns in CA1 pyramidal neurons of Wistar rats from 9 to 19 postnatal days (P9-19). The percentage of burst-spiking neurons increased up to P16, in correlation with the emergence of an active component in the ADP. The application of low-voltage-activated (LVA) calcium channel blockers such as nickel or mibefradil suppressed the generation of the active ADP component and burst-spiking behavior. In agreement with the development of the ADP waveform and burst-spiking behavior, voltage-clamp experiments in dissociated pyramidal neurons showed an increase in the LVA calcium current in P16-19 vs P9-12. Finally, we found that a reduction of extracellular calcium levels decreases the percentage of burst-spiking cells due to a reduction in the active component of the ADP. We conclude that a major contribution of LVA calcium channels to ADP determines the bursting capability of CA1 pyramidal neurons during a transitional postnatal period in contrast to adulthood. eng restricted access Role of low-voltage-activated calcium current and extracellular calcium in controlling the firing pattern of developing CA1 pyramidal neurons journal article