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oai:docta.ucm.es:20.500.14352/953752025-03-18T15:22:01Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Serafín González-Carrato, Verónica author Valverde De La Fuente, Alejandro author Martínez-García, Gonzalo author Martínez-Periñán, Emiliano author Comba, Fausto author Garranzo-Asensio, María author Barderas Manchado, Rodrigo author Yáñez-Sedeño Orive, Paloma author Campuzano Ruiz, Susana author Pingarrón Carrazón, José Manuel author 2019 In this work, the development of the first integrated electrochemical immunosensor for the determination of the IL-13Rα2 is reported. The strategy involves the immobilization of a biotinylated capture antibody onto streptavidin-modified screen-printed electrodes through grafting with p-aminobezoic acid (p-ABA) and further activation using EDC/Sulfo-NHS chemistry. A hybrid nanomaterial composed of multiwalled carbon nanotubes (MWCNTs) and Graphene Quantum Dots (GQDs) was used as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the system H2O2/hydroquinone (HQ) achieved a linear calibration plot ranging from 2.7 to 100 ng mL−1 IL-13sRα2, with a LOD value of 0.8 ng mL−1. The immunosensor showed an excellent selectivity and was successfully applied to the determination of the target receptor directly in small amounts of raw cellular lysates and extracts of paraffin-embedded tissues from patients diagnosed with colorectal cancer at different stages. Serafín V⁠, Valverde A⁠, Martínez-García G⁠, Martínez-Periñán E⁠, Comba F⁠, Garranzo-Asensio M⁠, Barderas R⁠, Yáñez-Sedeño P⁠, Campuzano S⁠, Pingarrón J.M. Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential. Sens. Actuators, B. 2019 Enero 3; 284: 711-22 0925-4005 10.1016/j.snb.2019.01.012 https://hdl.handle.net/20.500.14352/95375 https://doi.org/10.1016/j.snb.2019.01.012 https://produccioncientifica.ucm.es/grupos/4975/colaboracion/organizaciones/1230 Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential