2026-06-30T01:25:12Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/953752025-03-18T15:22:01Zcom_20.500.14352_14col_20.500.14352_15

Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential Serafín González-Carrato, Verónica Valverde De La Fuente, Alejandro Martínez-García, Gonzalo Martínez-Periñán, Emiliano Comba, Fausto Garranzo-Asensio, María Barderas Manchado, Rodrigo Yáñez-Sedeño Orive, Paloma Campuzano Ruiz, Susana Pingarrón Carrazón, José Manuel In this work, the development of the first integrated electrochemical immunosensor for the determination of the IL-13Rα2 is reported. The strategy involves the immobilization of a biotinylated capture antibody onto streptavidin-modified screen-printed electrodes through grafting with p-aminobezoic acid (p-ABA) and further activation using EDC/Sulfo-NHS chemistry. A hybrid nanomaterial composed of multiwalled carbon nanotubes (MWCNTs) and Graphene Quantum Dots (GQDs) was used as nanocarrier of multiple detector antibody and HRP molecules. Amperometric detection with the system H2O2/hydroquinone (HQ) achieved a linear calibration plot ranging from 2.7 to 100 ng mL−1 IL-13sRα2, with a LOD value of 0.8 ng mL−1. The immunosensor showed an excellent selectivity and was successfully applied to the determination of the target receptor directly in small amounts of raw cellular lysates and extracts of paraffin-embedded tissues from patients diagnosed with colorectal cancer at different stages. 2024-01-25T11:18:40Z 2024-01-25T11:18:40Z 2024-01-25T11:18:40Z 2019 journal article https://hdl.handle.net/20.500.14352/95375 0925-4005 10.1016/j.snb.2019.01.012 eng CTQ2015-70023-R CTQ2015-64402-C2-1R S2013/MT-3029 PI17CIII/00045 Serafín V⁠, Valverde A⁠, Martínez-García G⁠, Martínez-Periñán E⁠, Comba F⁠, Garranzo-Asensio M⁠, Barderas R⁠, Yáñez-Sedeño P⁠, Campuzano S⁠, Pingarrón J.M. Graphene quantum dots-functionalized multi-walled carbon nanotubes as nanocarriers in electrochemical immunosensing. Determination of IL-13 receptor α2 in colorectal cells and tumor tissues with different metastatic potential. Sens. Actuators, B. 2019 Enero 3; 284: 711-22 http://creativecommons.org/licenses/by-nc-nd/4.0/ open access Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier