2026-06-28T20:27:54Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/953872025-03-18T12:00:13Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Serafín González-Carrato, Verónica author Valverde De La Fuente, Alejandro author Garranzo-Asensio, María author Barderas Manchado, Rodrigo author Campuzano Ruiz, Susana author Yáñez-Sedeño Orive, Paloma author Pingarrón Carrazón, José Manuel author 2019 This paper describes a dual electrochemical immunoassay for the simultaneous determination of IL-13Rα2 and CDH-17, two biomarkers of emerging relevance in metastatic processes. The sandwich assay uses a screen-printed dual carbon electrode that was electrochemically grafted with p-aminobenzoic acid to allow the covalent immobilization of capture antibodies. A hybrid composed of graphene quantum dots (GQDs) and multiwalled carbon nanotubes (MWCNTs) act as nanocarriers for the detection antibodies and horseradish peroxidase. The use of this hybrid material considerably improves the assay (in comparison to the use of MWCNTs) due to the peroxidase mimicking activity of the GQDs. The method works at a low working potential (0.20 V vs. Ag pseudo-reference electrode) and thus is not readily interfered by unknown electroactive species. The dual immunoassay allows for the selective determination of both biomarkers with LOD values of 1.4 (IL-13sRα2) and 0.03 ng mL-1 (CDH-17). The simultaneous determination of IL-13Rα2 and CDH-17 was accomplished in lysates from breast and colorectal cancer cells with different metastatic potential, and in paraffin-embedded tumor tissues extracts from patients diagnosed with colorectal cancer at different stages. The applicability to discriminate the metastatic potential even in intact cells through the detection of both extracellular receptors has been demonstrated also. The assay can be performed within 3 h, requires small sample amounts (0.5 μg), and has a simple protocol. Graphical abstract Dual amperometric immunosensing of the metastasis-related biomarkers IL-13Rα2 and CDH-17 in human colorectal cancer cells and tissues by using grafted screen-printed electrodes and composites of quantum dots and carbon nanotubes as nanocarriers. Serafín V, Valverde A, Garranzo-Asensio M, Barderas R, Campuzano S, Yáñez-Sedeño P, Pingarrón J.M. Simultaneous determination of the emerging metastatic biomarkers IL-13Rα2 and CDH-17 using amperometric immunosensors involving grafted screen-printed electrodes and quantum dots/carbon nanotubes as carrier tags for signal amplification. Microchim. Acta. 2019 junio 10; 186(7):411-21 0026-3672 10.1007/s00604-019-3531-5 https://hdl.handle.net/20.500.14352/95387 1436-5073 https://doi.org/10.1007/s00604-019-3531-5 https://pubmed.ncbi.nlm.nih.gov/31183566/ Simultaneous amperometric immunosensing of the metastasis-related biomarkers IL-13Rα2 and CDH-17 by using grafted screen-printed electrodes and a composite prepared from quantum dots and carbon nanotubes for signal amplification