2026-06-29T16:49:18Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/954442024-07-18T23:53:35Zcom_20.500.14352_14col_20.500.14352_15
Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform
Torrente Rodríguez, Rebeca Magnolia
Campuzano Ruiz, Susana
Ruiz Valdepeñas Montiel, Víctor
Pedrero Muñoz, María
Fernández Aceñero, María Jesús
Barderas Manchado, Rodrigo
Pingarrón Carrazón, José Manuel
A sensitive and rapid method for the determination of the clinically relevant biomarker human endoglin (CD105) in serum samples is presented, involving a magneto-actuated immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs). Micro-sized magnetic particles were modified with a specific antibody to selectively capture the target protein which was further sandwiched with a secondary HRP-labeled antibody. The immunocomplexes attached to the magnetic carriers were amperometrically detected at SPCEs using the hydroquinone (HQ)/H2O2/HRP system. The magneto-actuated immunosensing platform was able to detect 5 pmoles of endoglin (in 25μL of sample, 0.2μM) in 30min providing statistically similar results to those obtained using a commercial ELISA kit for the determination of endogenous content of endoglin in human serum samples.
2024-01-25T12:53:18Z
2024-01-25T12:53:18Z
2024-01-25T12:53:18Z
2016
journal article
https://hdl.handle.net/20.500.14352/95444
0731-7085
10.1016/j.jpba.2016.07.020
eng
CTQ2015-64402-C2-1-R
S2013/MT–3029
Torrente-Rodríguez RM, Campuzano S, Ruiz-Valdepeñas-Montiel V, Pedrero M, Fernández-Aceñero MJ, Barderas R, Pingarrón JM. Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform. J Pharm Biomed Anal. 2016 Sep 10;129:288-293. doi: 10.1016/j.jpba.2016.07.020. Epub 2016 Jul 15. PMID: 27448312.
restricted access
Elsevier