2026-06-29T16:48:45Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/954442024-07-18T23:53:35Zcom_20.500.14352_14col_20.500.14352_15

Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform Torrente Rodríguez, Rebeca Magnolia Campuzano Ruiz, Susana Ruiz Valdepeñas Montiel, Víctor Pedrero Muñoz, María Fernández Aceñero, María Jesús Barderas Manchado, Rodrigo Pingarrón Carrazón, José Manuel A sensitive and rapid method for the determination of the clinically relevant biomarker human endoglin (CD105) in serum samples is presented, involving a magneto-actuated immunoassay and amperometric detection at disposable screen-printed carbon electrodes (SPCEs). Micro-sized magnetic particles were modified with a specific antibody to selectively capture the target protein which was further sandwiched with a secondary HRP-labeled antibody. The immunocomplexes attached to the magnetic carriers were amperometrically detected at SPCEs using the hydroquinone (HQ)/H2O2/HRP system. The magneto-actuated immunosensing platform was able to detect 5 pmoles of endoglin (in 25μL of sample, 0.2μM) in 30min providing statistically similar results to those obtained using a commercial ELISA kit for the determination of endogenous content of endoglin in human serum samples. 2024-01-25T12:53:18Z 2024-01-25T12:53:18Z 2016 journal article Torrente-Rodríguez RM, Campuzano S, Ruiz-Valdepeñas-Montiel V, Pedrero M, Fernández-Aceñero MJ, Barderas R, Pingarrón JM. Rapid endoglin determination in serum samples using an amperometric magneto-actuated disposable immunosensing platform. J Pharm Biomed Anal. 2016 Sep 10;129:288-293. doi: 10.1016/j.jpba.2016.07.020. Epub 2016 Jul 15. PMID: 27448312. 0731-7085 10.1016/j.jpba.2016.07.020 https://hdl.handle.net/20.500.14352/95444 https://doi.org/10.1016/j.jpba.2016.07.020 eng CTQ2015-64402-C2-1-R S2013/MT–3029 restricted access Elsevier