2026-06-08T01:06:04Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/955082025-03-18T13:15:22Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense
author
Wu, Dan
author
Cheung, Shane
author
Durán Sampedro, Gonzalo
author
Chen, Zhi-Long
author
Cahill, Ronan
author
O'Shea, Donal
2024-01-25T15:02:21Z
2024-01-25T15:02:21Z
2018
Wu, Dan, et al. «A DIE Responsive NIR-Fluorescent Cell Membrane Probe». Biochimica et Biophysica Acta (BBA) - Biomembranes, vol. 1860, n.o 11, noviembre de 2018, pp. 2272-80. https://doi.org/10.1016/j.bbamem.2018.09.006.
0005-2736
10.1016/j.bbamem.2018.09.006
https://hdl.handle.net/20.500.14352/95508
https://doi.org/10.1016/j.bbamem.2018.09.006
It is challenging to achieve selective off to on modulation of the emissive state of a fluorophore within a complex and heterogeneous cellular environment. Herein we show that the dis-assembly of a non-fluorescent aggregate to produce individual fluorescent molecules, termed disaggregation induced emission (DIE), can be utilised to achieve this goal with an amphiphilic BF2-azadipyrromethene (NIR-AZA) probe. Optical near-infrared properties of the NIR-AZA probe used in this study include absorption and emission maxima at 700 and 726 nm respectively when in the emissive non-aggregated state. Key to the success of the probe is the bis-sulfonic acid substitution of the NIR-AZA fluorophore, which is atypical for membrane probes as it does not contain zwitterionic lipid substituents. The aggregation/disaggregation properties of the NIR-fluorophore have been investigated in model surfactant and synthetic liposomal systems and shown to be emissive responsive to both. Real-time live cell imaging experiments in HeLa Kyoto and MC3T3 cells showed a rapid switch on of emission specific to the plasma membrane of viable and apoptotic cells attributable to a disaggregation-induced emission of the probe. Image analysis software confirmed localisation of fluorescence to the plasma membrane. Cell membrane staining was also effective for formaldehyde fixed cells, with staining possible either before or after fixation. This study adds new and important findings to recent developments of DIE responsive probes and further applications of this controllable emission-switching event are anticipated.
eng
A DIE responsive NIR-fluorescent cell membrane probe
journal article
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A_DIE_responsive_NIR-fluorescent_cell.pdf.txt