2026-06-27T12:39:19Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/956072024-10-02T17:18:25Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Martínez Orts, Mónica author Rubio-Somoza, I. author Díaz, I. author Mendez, S. author Alunda Rodríguez, José María author Ordóñez Gutiérrez, Lara 2024-01-25T19:00:29Z 2024-01-25T19:00:29Z 2009-12 Ordóñez-Gutiérrez L, Martínez M, Rubio-Somoza I, Díaz I, Mendez S, Alunda JM. Leishmania infantum: antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR. Exp Parasitol. 2009 Dec;123(4):341-6. doi: 10.1016/j.exppara.2009.08.015. Epub 2009 Aug 31. PMID: 19723520. 0014-4894 10.1016/j.exppara.2009.08.015 https://hdl.handle.net/20.500.14352/95607 https://www.sciencedirect.com/science/article/pii/S0014489409002525?via%3Dihub Two recombinant barley cystatins, HvCPI5 and HvCPI6, have been tested in vitro against promastigotes and intracellular amastigotes of Leishmania infantum in the J774 monocytic cell line. Toxicity of cystatins for J774 cells was also determined. In addition, a comparison between direct counts of intracellular amastigotes and quantitation of burden by Q-PCR was carried out. Low concentrations (2 lM) from both cystatins were unable to inhibit promastigote replication. HvCPI5 was toxic for mammalian cells; 0.1 lM reduced by more than 50% the cell viability. On the contrary, HvCPI6 did not exhibit any toxicity for J774 cells up to 6 lM and inhibited the intracellular amastigote multiplication. Dose–response analysis showed that 4.8 lM HvCPI6 reduced by >90% the intracellular parasite load and had an approximate IC50 value of 1.5 lM. Comparable results were obtained by direct counting of intracellular amastigotes and Q-PCR. Results point towards the direct inhibition of amastigote multiplication by HvCPI6 and the interest of this recombinant cystatin in the chemotherapy of leishmaniasis. eng Attribution-NonCommercial-NoDerivatives 4.0 International Leishmania infantum: Antiproliferative effect of recombinant plant cystatins on promastigotes and intracellular amastigotes estimated by direct counting and real-time PCR journal article 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URL https://docta.ucm.es/bitstreams/7232279b-3cd7-4f0e-a354-3998a7f14b29/download File MD5 3cfb83cd3b206d45cc360cf146b360b9 332301 application/pdf leishmania infantum.pdf