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oai:docta.ucm.es:20.500.14352/957482024-09-03T23:54:03Zcom_20.500.14352_14col_20.500.14352_15

Prem, Deepak Solís González, María Teresa Bárány, Ivett Rodríguez-Sanz, Héctor Risueño, María del Carmen Testillano, Pilar 2024-01-29T08:51:27Z 2024-01-29T08:51:27Z 2012 Prem, D., Solís, MT., Bárány, I. et al. A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus. BMC Plant Biol 12, 127 (2012). https://doi.org/10.1186/1471-2229-12-127 1471-2229 https://hdl.handle.net/20.500.14352/95748 https://doi.org/10.1186/1471-2229-12-127 Microspore embryogenesis represents a unique system of single cell reprogramming in plants wherein a highly specialized cell, the microspore, by specific stress treatment, switches its fate towards an embryogenesis pathway. In Brassica napus, a model species for this phenomenon, incubation of isolated microspores at 32°C is considered to be a pre-requisite for embryogenesis induction. eng http://creativecommons.org/licenses/by/4.0/ open access Attribution 4.0 International A new microspore embryogenesis system under low temperature which mimics zygotic embryogenesis initials, expresses auxin and efficiently regenerates doubled-haploid plants in Brassica napus journal article