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00925njm 22002777a 4500 dc Broide, David H. author Stachnick, Greg author Castaneda, Diego author Nayar, Jyothi author Miller, Marina author Cho, Jae Youn author Roman, Mark author Zubeldia Ortuño, José Manuel author Hyashi, Tomoko author Raz, Eyal author 2001-05-01 This study investigated whether immunostimulatory DNA sequences (ISS) induce a transient or sustained inhibition of Th2 responses to inhaled antigen. We sensitized mice with subcutaneous injections to develop a Th2 response to ovalbumin (ova) and then administered a dose of ISS prior to ova inhalation challenge. Mice were then rechallenged with ova by inhalation a second time at varying time points after the first ova inhalation (1 to 8 weeks later) to determine whether the ISS dose administered prior to the first ova inhalation protected against a subsequent second ova inhalation challenge. A single dose of ISS inhibited the Th2 response to the first inhalation of ova antigen, as well as 4 weeks later to the second inhalation of ova. However, ISS did not inhibit a Th2 response to the second inhalation of ova 8 weeks later. The reversible inhibition of Th2 responses at 8 weeks suggests the need for repeated ISS administration at monthly intervals. Broide DH, Stachnick G, Castaneda D, Nayar J, Miller M, Cho JY, Roman M, Zubeldia J, Hayashi T, Raz E. Systemic administration of immunostimulatory DNA sequences mediates reversible inhibition of Th2 responses in a mouse model of asthma. Actions Share Page navigation Title & authors Erratum in Abstract Similar articles Cited by References Publication types MeSH terms Substances Related information Grants and funding LinkOut - more resources J Clin Immunol . 2001 May;21(3):175-82. 0271-9142 10.1023/a:1011078930363 https://hdl.handle.net/20.500.14352/95765 https://link.springer.com/article/10.1023/A:1011078930363 https://pubmed.ncbi.nlm.nih.gov/11403224/ Systemic administration of immunostimulatory DNA sequences mediates reversible inhibition of Th2 responses in a mouse model of asthma