2026-06-08T00:03:44Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/957822024-09-17T23:49:50Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Díaz Mendoza, María Mercedes author Dominguez-Figueroa, Jose author Velasco-Arroyo, Blanca author Cambra, Ines author Gonzalez-Melendi, Pablo author Lopez-Gonzalvez, Angeles author Garcia, Antonia author Hensel, Goetz author Kumlehn, Jochen author Diaz, Isabel author Martinez, Manuel author 2016 Proteolysis is an essential process throughout the mobilization of storage proteins in barley (Hordeum vulgare) grains during germination. It involves numerous types of enzymes, with C1A Cys proteases the most abundant key players. Manipulation of the proteolytic machinery is a potential way to enhance grain yield and quality, and it could influence the mobilization of storage compounds along germination. Transgenic barley plants silencing or over-expressing the cathepsin F-like HvPap-1 Cys protease show differential accumulation of storage molecules such as starch, proteins, and free amino acids in the grain. It is particularly striking that the HvPap-1 artificial microRNA lines phenotype show a drastic delay in the grain germination process. Alterations to the proteolytic activities in the over-expressing and knock-down grains associated with changes in the level of expression of several C1A peptidases were also detected. Similarly, down-regulating cystatin Icy-2, one of the proteinaceous inhibitors of the cathepsin F-like protease, also has important effects on grain filling. However, the ultimate physiological influence of manipulating a peptidase or an inhibitor cannot be always predicted, since the plant tries to compensate the modified proteolytic effects by modulating the expression of some other peptidases or their inhibitors. Mercedes Diaz-Mendoza, Jose D. Dominguez-Figueroa, Blanca Velasco-Arroyo, Ines Cambra, Pablo Gonzalez-Melendi, Angeles Lopez-Gonzalvez, Antonia Garcia, Goetz Hensel, Jochen Kumlehn, Isabel Diaz, Manuel Martinez, HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination, Plant Physiology, Volume 170, Issue 4, April 2016, Pages 2511–2524, https://doi.org/10.1104/pp.15.01944 0032-0889 10.1104/pp.15.01944 https://hdl.handle.net/20.500.14352/95782 1532-2548 https://doi.org/10.1104/pp.15.01944 HvPap-1 C1A Protease and HvCPI-2 Cystatin Contribute to Barley Grain Filling and Germination