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00925njm 22002777a 4500 dc Mejías, Raquel author Costo, Rocío author Roca, Alejandro G. author Fernández Arias, Cristina author Veintemillas-Verdaguer, S. author González-Carreño, T. author Puerto Morales, M. del author Serna, C.J. author Mañes, S. author Barber, D.F. author 2008-09-10 Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases. 0168-3659 10.1016/j.jconrel.2008.05.028 https://hdl.handle.net/20.500.14352/96469 1873-4995 https://www.sciencedirect.com/science/article/abs/pii/S0168365908003301?via%3Dihub Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery