2026-06-27T10:49:26Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/964692025-03-18T14:23:24Zcom_20.500.14352_14col_20.500.14352_15
Mejías, Raquel
Costo, Rocío
Roca, Alejandro G.
Fernández Arias, Cristina
Veintemillas-Verdaguer, S.
González-Carreño, T.
Puerto Morales, M. del
Serna, C.J.
Mañes, S.
Barber, D.F.
2024-01-30T12:16:59Z
2024-01-30T12:16:59Z
2008-09-10
0168-3659
10.1016/j.jconrel.2008.05.028
https://hdl.handle.net/20.500.14352/96469
1873-4995
https://www.sciencedirect.com/science/article/abs/pii/S0168365908003301?via%3Dihub
Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases.
eng
restricted access
Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery
journal article