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oai:docta.ucm.es:20.500.14352/964692025-03-18T14:23:24Zcom_20.500.14352_14col_20.500.14352_15

Cytokine adsorption/release on uniform magnetic nanoparticles for localized drug delivery Mejías, Raquel Costo, Rocío Roca, Alejandro G. Fernández Arias, Cristina Veintemillas-Verdaguer, S. González-Carreño, T. Puerto Morales, M. del Serna, C.J. Mañes, S. Barber, D.F. Attachment of cytokines to magnetic nanoparticles has been developed as a system for controlled local drug release in cancer therapy. We studied the adsorption/release of murine interferon gamma (IFN-gamma) on negatively charged magnetic nanoparticles prepared by three different methods, including coprecipitation, decomposition in organic media, and laser pyrolysis. To facilitate IFN-gamma adsorption, magnetic nanoparticles were surface modified by distinct molecules to achieve high negative charge at pH 7, maintaining small aggregate size and stability in biological media. We analyzed carboxylate-based coatings and studied the colloidal properties of the resulting dispersions. Finally, we incubated the magnetic dispersions with IFN-gamma and determined optimal conditions for protein adsorption onto the particles, as well as the release capacity at different pH and as a function of time. Particles prepared by decomposition in organic media and further modified with dimercaptosuccinic acid showed the most efficient adsorption/release capacity. IFN-gamma adsorbed on these nanoparticles would allow concentration of this protein or other biomolecules at specific sites for treatment of cancer or other diseases. 2024-01-30T12:16:59Z 2024-01-30T12:16:59Z 2008-09-10 journal article 0168-3659 10.1016/j.jconrel.2008.05.028 https://hdl.handle.net/20.500.14352/96469 1873-4995 https://www.sciencedirect.com/science/article/abs/pii/S0168365908003301?via%3Dihub eng restricted access Elsevier