2026-06-29T02:42:11Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/966152025-03-18T13:59:09Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Boujbiha, Mohamed Ali author Chahdoura, Hassiba author Adouni, Khaoula author Ziani, Borhane Eddine Cherif author Snoussi, Mejdi author Chakroun, Yasmine author Ciudad Mulero, María author Fernández Ruiz, Virginia author Achour, Lotfi author Selmi, Boulbaba author Morales Gómez, Patricia author Flamini, Guido author 2023-06-29 Wild Vitex agnus-castus (VAC) is a Mediterranean plant that is rich in bioactive metabolites. This study aimed to validate, for the first time, the beneficial use of VAC fruits and fruit decoctions (VFDs) through in vitro and in vivo trials. Forty-one volatile components were detected in VAC fruits, with 1,8-cineole (30.3%) comprising the majority. The antioxidant activity of VFD was measured by using different in vitro methods (EC50 of 0.16 mg/mL by β-carotene bleaching inhibition assay) and by measuring the DNA protection power. Using the disc diffusion assay, the antimicrobial activity of VFD was evaluated, and it exhibited a noticeable anticandidal activity. VFD did not cause any toxicity or mortality in rats treated with doses > 200 mg/kg. Using the acetic acid writhing test, the antinociceptive activity of VFD was measured. Our results showed that VFD at 200 mg/kg exhibited a higher analgesic activity (81.68%) than acetylsalicylic acid used as a positive control (74.35%). Its gastroprotective ability was assessed by HCl/ethanol-induced gastric lesions, which were remarkably inhibited (84.62%) by intraperitoneal administration of VFD. This work helps to validate the popular use of VAC to treat nociceptive, inflammatory, and gastric disorders and encourages researchers to further investigate the identification of pharmacological compounds from this species. Boujbiha MA, Chahdoura H, Adouni K, Ziani BEC, Snoussi M, Chakroun Y, et al. Wild Vitex agnus-castus L.: Phytochemical Characterization, Acute Toxicity, and Bioactive Properties. Molecules 2023;28:5096. https://doi.org/10.3390/molecules28135096. 10.3390/molecules28135096 https://hdl.handle.net/20.500.14352/96615 https://doi.org/10.3390/molecules28135096 Wild Vitex agnus-castus L.: phytochemical characterization, acute toxicity, and bioactive properties