2026-06-28T15:00:03Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/967422025-04-01T00:07:23Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Fernández-Criado, Carmen author Martos-Rodríguez, Antonia author Santos Álvarez, Inmaculada author García-Ruíz, Josefa Predestinación author Delgado-Baeza, Emilio author 2004 Objective: The aim of the present study was to demonstrate whether bGH transgenic mice develop OA. We therefore studied in this animal model the structural features of cartilage and the subchondral bone changes of the knee joints that may be associated with osteoarthritic lesion. Method: Degenerative changes in the knee joints of bGH transgenic female mice (N = 11) and control mice (N = 11) were histologically analyzed at the age of 7 months. Histochemical and stereological studies were conducted. Immunohistochemistry on cell cyclin activity (assessed by anti-PCNA labeling) and cell viability (assessed by bcl-2 expression), as well as ribosomal activity (AgNOR), TNF-alpha expression and apoptosis (TUNEL technique) were performed. In ten 7-month-old female mice (Tg+ N = 5; control N = 5) the knee articular cartilages were studied with electron microscopy techniques. Results: Disruption of the articular surface (18.2%), cleft (63.7%), cloning (81.8%), hypocellularity of chondrocytes (18.2%), moderate (54.6%) to severe (45.4%) loss of safranin-O staining, and duplication and rupture of the tidemark (54.5%) were some of the main features observed in articular cartilage chondrocytes of bGH transgenic mice. Furthermore, cell cyclin activity and cell viability decreased, while TNF-alpha expression and TUNEL+ cells increased. These chondrocytes also showed an increase in the number of black dots per cell, as revealed by the AgNOR technique. Conclusion: Our results show that bGH transgenic mice develop a lesion of the articular cartilage consistent with that described in osteoarthritis. Fernández-Criado, C., Martos-Rodríguez, A., Santos-Alvarez, I., García-Ruíz, J. P., & Delgado-Baeza, E. (2004). The fate of chondrocyte in osteoarthritic cartilage of transgenic mice expressing bovine GH. Osteoarthritis and cartilage, 12(7), 543–551. https://doi.org/10.1016/j.joca.2004.04.002 1063-4584 10.1016/j.joca.2004.04.002 https://hdl.handle.net/20.500.14352/96742 https://doi.org/10.1016/j.joca.2004.04.002 15219569 The fate of chondrocyte in osteoarthritic cartilage of transgenic mice expressing bovine GH