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00925njm 22002777a 4500 dc George, Susan author Baranyi, József author García De Fernando Minguillón, Gonzalo Doroteo author Velasco De Diego, Raquel author Pin Arias, Carmen author 2004 Specific growth and death rates of Aeromonas hydrophila were measured in laboratory media under various combinations of temperature, pH, and percent CO2 and O2 in the atmosphere. Predictive models were developed from the data and validated by means of observations obtained from (i) seafood experiments set up for this purpose and (ii) the ComBase database (http://www.combase.cc; http://wyndmoor.arserrc.gov/combase/). Two main reasons were identified for the differences between the predicted and observed growth in food: they were the variability of the growth rates in food and the bias of the model predictions when applied to food environments. A statistical method is presented to quantitatively analyze these differences. The method was also used to extend the interpolation region of the model. In this extension, the concept of generalized Z values (C. Pin, G. García de Fernando, J. A. Ordo´n˜ez, and J. Baranyi, Food Microbiol. 18:539-545, 2001) played an important role. The extension depended partly on the density of the model-generating observations and partly on the accuracy of extrapolated predictions close to the boundary of the interpolation region. The boundary of the growth region of the organism was also estimated by means of experimental results for growth and death rates. Pin C, Velasco de Diego R, George S, García de Fernando GD, Baranyi J. Analysis and Validation of a Predictive Model for Growth and Death of Aeromonas hydrophila under Modified Atmospheres at Refrigeration Temperatures. Appl Environ Microbiol. 2004 Jul;70(7):3925-32 0099-2240 10.1128/AEM.70.7.3925–3932.2004 https://hdl.handle.net/20.500.14352/96755 https://www.doi.org/10.1128/AEM.70.7.3925-3932.2004 https://journals.asm.org/doi/10.1128/aem.70.7.3925-3932.2004 Analysis and validation of a predictive model for growth and death of Aeromonas hydrophila under modified atmospheres at refrigeration temperatures