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00925njm 22002777a 4500 dc Andrés-Delgado,Laura author Antón Hurtado, Olga María author Bartolini, Francesca author Ruiz-Sáenz, Ana author Correas, Isabel author Gundersen, Gregg author Alonso, Miguel author 2012 T cell antigen receptor–proximal signaling components, Rho-family GTPases, and formin proteins DIA1 and FMNL1 have been implicated in centrosome reorientation to the immunological synapse of T lymphocytes. However, the role of these molecules in the reorientation process is not yet defined. Here we find that a subset of microtubules became rapidly stabilized and that their α-tubulin subunit posttranslationally detyrosinated after engagement of the T cell receptor. Formation of stabilized, detyrosinated microtubules required the formin INF2, which was also found to be essential for centrosome reorientation, but it occurred independently of T cell receptor–induced massive tyrosine phosphorylation. The FH2 domain, which was mapped as the INF2 region involved in centrosome repositioning, was able to mediate the formation of stable, detyrosinated microtubules and to restore centrosome translocation in DIA1-, FMNL1-, Rac1-, and Cdc42-deficient cells. Further experiments indicated that microtubule stabilization was required for centrosome polarization. Our work identifies INF2 and stable, detyrosinated microtubules as central players in centrosome reorientation in T cells. Laura Andrés-Delgado, Olga M. Antón, Francesca Bartolini, Ana Ruiz-Sáenz, Isabel Correas, Gregg G. Gundersen, Miguel A. Alonso; INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells. J Cell Biol 17 September 2012; 198 (6): 1025–1037. doi: https://doi.org/10.1083/jcb.201202137 1540-8140 10.1083/jcb.201202137 https://hdl.handle.net/20.500.14352/97002 0021-9525 https://doi.org/10.1083/jcb.201202137 INF2 promotes the formation of detyrosinated microtubules necessary for centrosome reorientation in T cells