2026-06-07T15:18:13Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/972572024-09-25T00:09:23Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Fernández Messina, Lola María author Ashiru, Omodele author Agüera-González, Sonia author Reyburn, Hugh author Valés-Gómez, Mar 2024-01-31T14:46:21Z 2024-01-31T14:46:21Z 2011 Lola Fernández-Messina, Omodele Ashiru, Sonia Agüera-González, Hugh T. Reyburn, Mar Valés-Gómez; The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells. J Cell Sci 1 February 2011; 124 (3): 321–327. doi: https://doi.org/10.1242/jcs.076042 0021-9533 10.1242/jcs.076042 https://hdl.handle.net/20.500.14352/97257 1477-9137 https://doi.org/10.1242/jcs.076042 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3021996/ The activating immune receptor NKG2D binds to several stress-induced ligands that are structurally different. MHC-class-I-related chain (MIC) A/B molecules have a transmembrane domain, whereas most UL16 binding proteins (ULBPs) are glycosylphosphatidylinositol (GPI)-linked molecules. The significance of this variability in membrane anchors is unclear. Here, we demonstrate that ULBP2, but not ULBP1 or ULBP3, can reach the cell surface without the GPI modification. Several proteins are expressed at the cell surface as both transmembrane and GPI-linked molecules, either via alternative splicing or by the expression of linked genes. However, to our knowledge, ULBP2 is the first single mammalian cDNA that can be expressed as either a transmembrane or a GPI-anchored protein. The rate of maturation and the levels of cell surface expression of the non-GPI-linked form were lower than those of the GPI-linked ULBP2. Nonetheless, non-GPI ULBP2 was recognised by NKG2D and triggered NK cell cytotoxicity. These data show that differences in membrane attachment by NKG2D ligands are more important for regulation of their surface expression than for cytotoxic recognition by NKG2D and emphasise that detailed characterisation of the cell biology of individual NKG2D ligands will be necessary to allow targeted modulation of this system. eng The human NKG2D ligand ULBP2 can be expressed at the cell surface with or without a GPI anchor and both forms can activate NK cells journal article 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URL https://docta.ucm.es/bitstreams/2d465d76-2ea7-45fb-acc9-3625150de8f2/download File MD5 4c5fba4f6cb6c620aa30e96718dc22e5 666479 application/pdf Human_NKG2D_ligand_ULBP2.pdf