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00925njm 22002777a 4500 dc Gosálvez Berenguer, Jaime author Crespo Castejón, Francisco author Gutiérrez Cepeda, Luna author Serres Dalmau, María Consolacion author Jonhnston, Stephen author 2013 Stallion spermatozoa recovered and examined immediatelyafter colloidal centrifugation resulted in a higher straight-linevelocity (VSL) than sperm processed using direct conven-tional centrifugation (p = 0.000), but there was nodifferences in the progressive motility or sperm DNAfragmentation (SDF) as determined by the sperm chromatindispersion assay. However, when centrifuged spermatozoawere incubated at 37°C for 24 h to determine the rate of SDF(r-SDF), a lower r-SDF (p = 0.0011) was observed in thosesperm recovered after colloidal separation (0.5 ± 0.1%⁄h)compared to direct (1.2 ± 0.4%⁄h) or no centrifugation (r-SDF = 1.2 ± 0.3%⁄h). These results confirm that colloidalseparation of stallion spermatozoa results in prolonged spermDNA longevity, but these differences were only apparentfollowing a period of incubation and dynamic assessment.Consequently, we strongly recommend the use of thedynamic form of the SDF assay for evaluating centrifugationand⁄or otherex vivoprocedures, as a single basal assessmentof SDF may inadvertently result in a false-positive evaluationof DNA quality. Crespo F, Gosalvez J, Gutiérrez-Cepeda L, Serres C, Johnston SD. Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation. Reprod Domest Anim. 2013 Apr;48(2):e23-5. 0936-6768 10.1111/j.1439-0531.2012.02140.x https://hdl.handle.net/20.500.14352/97263 https://doi.org/10.1111/j.1439-0531.2012.02140.x 22775967 Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation