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oai:docta.ucm.es:20.500.14352/972632025-04-01T00:08:33Zcom_20.500.14352_14col_20.500.14352_15

Gosálvez Berenguer, Jaime Crespo Castejón, Francisco Gutiérrez Cepeda, Luna Serres Dalmau, María Consolacion Jonhnston, Stephen 2024-01-31T14:57:15Z 2024-01-31T14:57:15Z 2013 Crespo F, Gosalvez J, Gutiérrez-Cepeda L, Serres C, Johnston SD. Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation. Reprod Domest Anim. 2013 Apr;48(2):e23-5. 0936-6768 10.1111/j.1439-0531.2012.02140.x https://hdl.handle.net/20.500.14352/97263 https://doi.org/10.1111/j.1439-0531.2012.02140.x 22775967 Stallion spermatozoa recovered and examined immediatelyafter colloidal centrifugation resulted in a higher straight-linevelocity (VSL) than sperm processed using direct conven-tional centrifugation (p = 0.000), but there was nodifferences in the progressive motility or sperm DNAfragmentation (SDF) as determined by the sperm chromatindispersion assay. However, when centrifuged spermatozoawere incubated at 37°C for 24 h to determine the rate of SDF(r-SDF), a lower r-SDF (p = 0.0011) was observed in thosesperm recovered after colloidal separation (0.5 ± 0.1%⁄h)compared to direct (1.2 ± 0.4%⁄h) or no centrifugation (r-SDF = 1.2 ± 0.3%⁄h). These results confirm that colloidalseparation of stallion spermatozoa results in prolonged spermDNA longevity, but these differences were only apparentfollowing a period of incubation and dynamic assessment.Consequently, we strongly recommend the use of thedynamic form of the SDF assay for evaluating centrifugationand⁄or otherex vivoprocedures, as a single basal assessmentof SDF may inadvertently result in a false-positive evaluationof DNA quality. eng restricted access Colloidal centrifugation of stallion semen results in a reduced rate of sperm DNA fragmentation journal article