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oai:docta.ucm.es:20.500.14352/972922025-03-18T12:03:42Zcom_20.500.14352_14col_20.500.14352_15

Hidalgo Prieto, Manuel Ortiz Jaraba, Isabel Dorado Martín, Jesús Morrel, Jane M Gosálvez Berenguer, Jaime Consuegra González, Cesar Diaz Jimenez, María Pereira Aguilar, Blasa Crespo Castejón, Francisco 2024-01-31T15:38:00Z 2024-01-31T15:38:00Z 2017 Hidalgo M, Ortiz I, Dorado J, Morrell JM, Gosálvez J, Consuegra C, Diaz-Jimenez M, Pereira B, Crespo F. Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation. Anim Reprod Sci. 2017 Oct;185:83-88 0378-4320 10.1016/j.anireprosci.2017.08.005 https://hdl.handle.net/20.500.14352/97292 1873-2232 https://doi.org/10.1016/j.anireprosci.2017.08.005 28827080 http://www.journals.elsevier.com/animal-reproduction-science/ The aims of this study were to: 1) develop a new method for stallion sperm selection using a modified swim-up procedure through a colloid and 2) evaluate its impact in good quality eja-culates from bad freezers in comparison to methods involving centrifugation such as single layer centrifugation and sperm washing. Ejaculates were processed before freezing using three dif-ferent procedures: sperm washing (SW), colloid single layer centrifugation (SLC) and a modified colloid swim-up (SU). After semen processing, sperm recovery rates were measured and sperm were frozen. Post-thaw sperm motility (assessed by computer-assisted sperm analysis), normal forms and plasma membrane integrity (evaluated under bright-field and fluorescence microscopy respectively), and DNA fragmentation (assessed by the Sperm-Halomax kit) were compared be-tween treatments. Sperm recovery rates were similar between SU and SLC but lower than SW. Sperm motility after thawing was lower in SU in comparison to SLC and SW, maybe due to the incomplete removal of seminal plasma before freezing. Sperm DNA fragmentation was lower in SU and SLC selection methods, particularly in SLC selected samples during the first 6 h of in-cubation. The remaining sperm parameters assessed were similar among treatments. In conclu-sion, SLC is more suitable than SW and SU to process stallion semen prior to freezing, in parti-cular when sperm DNA damage is suspected. Further studies are needed in order to determine the potential benefits of SU in samples where centrifugation is not necessary, such as epididymal sperm, ejaculate fractioning or post-thaw semen samples. eng http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International Stallion sperm selection prior to freezing using a modified colloid swim-up procedure without centrifugation journal article