2026-06-28T09:48:42Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/973782025-03-18T12:50:11Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Henriques, Dora author Browne, Keith author Barnett, Mark author Parejo, Melanie author Kryger, Per author Freeman, Tom author Muñoz Gabaldón, Irene author Garnery, Lionel author Highet, Fiona author Jonhston, Spencer author McCormack, Grace author Pinto, Alice author 2018 The natural distribution of the honeybee (Apis mellifera L.) has been changed by humans in recent decades to such an extent that the formerly widest-spread European subspecies, Apis mellifera mellifera, is threatened by extinction through introgression from highly divergent commercial strains in large tracts of its range. Conservation efforts for A. m. mellifera are underway in multiple European countries requiring reliable and cost-efficient molecular tools to identify purebred colonies. Here, we developed four ancestry-informative SNP assays for high sample throughput genotyping using the iPLEX Mass Array system. Our customized assays were tested on DNA from individual and pooled, haploid and diploid honeybee samples extracted from different tissues using a diverse range of protocols. The assays had a high genotyping success rate and yielded accurate genotypes. Performance assessed against whole-genome data showed that individual assays behaved well, although the most accurate introgression estimates were obtained for the four assays combined (117 SNPs). The best compromise between accuracy and genotyping costs was achieved when combining two assays (62 SNPs). We provide a ready-to-use cost-effective tool for accurate molecular identification and estimation of introgression levels to more effectively monitor and manage A. m. mellifera conservatories. Henriques, D., Browne, K.A., Barnett, M.W. et al. High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool. Sci Rep 8, 8552 (2018). https://doi.org/10.1038/s41598-018-26932-1 2045-2322 10.1038/s41598-018-26932-1 https://hdl.handle.net/20.500.14352/97378 https://doi.org/10.1038/s41598-018-26932-1 High sample throughput genotyping for estimating C-lineage introgression in the dark honeybee: an accurate and cost-effective SNP-based tool