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oai:docta.ucm.es:20.500.14352/973792025-03-04T00:53:52Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Cortés-Gutiérrez, Elva author Dávila-Rodríguez, Martha author López-Fernández, Carmen author Fernández, José Luis author Crespo Castejón, Francisco author Gosálvez Berenguer, Jaime author 2014 The presence of constitutive alkali-labile sites (ALS) has been investigated using a protocol of DNA breakage detection-fluorescence in situ hybridization and comet assay in spermatozoa of donkey (Equus asinus) and stallion (Equus caballus). These results were compared with those obtained using a similar experimental approach using somatic cells. The relative abundance of ALS was of the order of four times more in spermatozoa than in somatic cells. Alkali-labile sites showed a tendency to cluster localized at the equatorial-distal regions of the sperm. The amount of hybridized signal in the ALS in the sperm of donkey (Equus asinus) was 1.3 times greater than in stallion (Equus caballus), and the length of the comet tail obtained in donkey sperm was 1.6 times longer than that observed in stallion (P < 0.05); however, these differences were not appreciated in somatic cells. In conclusion, ALS localization in sperm is not a randomized event and a different pattern of ALS distribution occurs for each species. These results suggest that ALS represents a species-specific issue related to chromatin organization in sperm and somatic cells in mammalian species, and they might diverge even with very short phylogenetic distances. Cortés-Gutiérrez EI, Dávila-Rodríguez MI, López-Fernández C, Fernández JL, Crespo F, Gosálvez J. Localization of alkali-labile sites in donkey (Equus asinus) and stallion (Equus caballus) spermatozoa. Theriogenology. 2014 Jan 15;81(2):321-5. 0093-691X 10.1016/j.theriogenology.2015.03.021 https://hdl.handle.net/20.500.14352/97379 1879-3231 https://doi.org/10.1016/j.theriogenology.2013.10.001 24182740 http://www. sciencedirect. com/journal/theriogenology Localization of alkali-labile sites in donkey (Equus asinus) and stallion (Equus caballus) spermatozoa