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oai:docta.ucm.es:20.500.14352/974242025-03-04T00:47:22Zcom_20.500.14352_14col_20.500.14352_15

Sex-sorted bovine spermatozoa and DNA damage: I. Static features Gosálvez Berenguer, Jaime Ramírez, Miguel Ángel López Fernández, Carmen Crespo Castejón, Francisco Evans, Michael Kjelland, Michael Moreno, Juan Francisco This study examined the static response of Spermatozoa DNA Fragmentation (SDF) after sex selection in bulls using a MoFlo® SX (Beckman Coulter, Miami FL) spermatozoa sorter to produce three different subpopulations: 1) Spermatozoa bearing X- chromosomes with a purity of 95%, 2) Spermatozoa bearing Y-chromosomes with a purity of 95%, and 3) non-viable spermatozoa. The static response of SDF refers to the baseline values observed for DNA damage when analyzed pre- and post sex-sorting. Results showed that while the baseline level SDF in pre-sorted bull spermatozoa samples ranged from 5.3% to 11% with an average of 7.9% ± 2.1%, the level of SDF obtained in X- and Y-chromosome sorted samples was much lower (3.1% ± 1.9%) and statistical differences were obtained after comparing both groups (P < 0.01). Spermatozoa containing a fragmented DNA molecule tend to be accumulated in the non-viable subpopulation. The baseline SDF level in X- and Y-chromosome sorted subpopulations is reduced, by 63% on average when compared to the values obtained in the neat semen sample. Different bulls exhibit unique SDF reduction efficiencies via the X- and Y-chromosome sex selection process. 2024-01-31T19:40:22Z 2024-01-31T19:40:22Z 2011 journal article Gosálvez J, Ramirez MA, López-Fernández C, Crespo F, Evans KM, Kjelland ME, Moreno JF. Sex-sorted bovine spermatozoa and DNA damage: I. Static features. Theriogenology. 2011 Jan 15;75(2):197-205 0093-691X 10.1016/j.theriogenology.2010.08.006 https://hdl.handle.net/20.500.14352/97424 https://doi.org/10.1016/j.theriogenology.2010.08.006 20932559 https://pubmed.ncbi.nlm.nih.gov/20932559/ eng BFU 2007-66340/BFI http://creativecommons.org/licenses/by-nc-nd/4.0/ restricted access Attribution-NonCommercial-NoDerivatives 4.0 International Elsevier