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oai:docta.ucm.es:20.500.14352/975772025-03-18T13:14:55Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Haro-González, P. author Ramsay, W.T. author Martínez Maestro, Laura author Del Rosal, B. author Santacruz-Gomez, K. author Iglesias-De La Cruz, M.D.C. author Sanz-Rodríguez, F. author Chooi, J.Y. author Sevilla, P.R. author Bettinelli, M. author Choudhury, D. author Kar, A.K. author Solé, J.G. author Jaque, D. author Paterson, L. author 2013-02-11 Laser-induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 degrees C), thus, avoiding cell damage. Haro-González, P., Ramsay, W.T., Maestro, L.M., del Rosal, B., Santacruz-Gomez, K., del Carmen Iglesias-de la Cruz, M., Sanz-Rodríguez, F., Chooi, J.Y., Sevilla, P.R., Bettinelli, M., Choudhury, D., Kar, A.K., Solé, J.G., Jaque, D. and Paterson, L. (2013), Quantum Dot-Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells. Small, 9: 2162-2170. https://doi.org/10.1002/smll.201201740 1613-6810 10.1002/SMLL.201201740 https://hdl.handle.net/20.500.14352/97577 1613-6829 https://doi.org/10.1002/smll.201201740 Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells