2026-06-07T18:57:58Zhttps://docta.ucm.es/rest/oai/requestoai:docta.ucm.es:20.500.14352/975772025-03-18T13:14:55Zcom_20.500.14352_14col_20.500.14352_15
Haro-González, P.
Ramsay, W.T.
Martínez Maestro, Laura
Del Rosal, B.
Santacruz-Gomez, K.
Iglesias-De La Cruz, M.D.C.
Sanz-Rodríguez, F.
Chooi, J.Y.
Sevilla, P.R.
Bettinelli, M.
Choudhury, D.
Kar, A.K.
Solé, J.G.
Jaque, D.
Paterson, L.
2024-02-01T11:11:56Z
2024-02-01T11:11:56Z
2013-02-11
Haro-González, P., Ramsay, W.T., Maestro, L.M., del Rosal, B., Santacruz-Gomez, K., del Carmen Iglesias-de la Cruz, M., Sanz-Rodríguez, F., Chooi, J.Y., Sevilla, P.R., Bettinelli, M., Choudhury, D., Kar, A.K., Solé, J.G., Jaque, D. and Paterson, L. (2013), Quantum Dot-Based Thermal Spectroscopy and Imaging of Optically Trapped Microspheres and Single Cells. Small, 9: 2162-2170. https://doi.org/10.1002/smll.201201740
1613-6810
10.1002/SMLL.201201740
https://hdl.handle.net/20.500.14352/97577
1613-6829
https://doi.org/10.1002/smll.201201740
Laser-induced thermal effects in optically trapped microspheres and single cells are investigated by quantum dot luminescence thermometry. Thermal spectroscopy has revealed a non-localized temperature distribution around the trap that extends over tens of micrometers, in agreement with previous theoretical models besides identifying water absorption as the most important heating source. The experimental results of thermal loading at a variety of wavelengths reveal that an optimum trapping wavelength exists for biological applications close to 820 nm. This is corroborated by a simultaneous analysis of the spectral dependence of cellular heating and damage in human lymphocytes during optical trapping. This quantum dot luminescence thermometry demonstrates that optical trapping with 820 nm laser radiation produces minimum intracellular heating, well below the cytotoxic level (43 degrees C), thus, avoiding cell damage.
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Quantum dot-based thermal spectroscopy and imaging of optically trapped microspheres and single cells
journal article