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oai:docta.ucm.es:20.500.14352/976162025-03-31T23:57:18Zcom_20.500.14352_14col_20.500.14352_15

00925njm 22002777a 4500 dc Martínez Caballero, María Aranzazu author Ares Lombán, Irma author Martínez Caballero, Marta author López Torres, Bernardo author Rodríguez, José Luis author Maximiliano Guerra, Jorge Enrique author Martínez Larrañaga, María Rosa author Anadón Navarro, Arturo Ramón author Rosa, José Manuel de la author Cueto, Mercedes author 2020 The present study investigated the effect of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) on cell viability and the potential protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butyl hydroperoxide (t-BOOH). Both extracts prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with t-BOOH. Increased ROS and caspase 3/7 activity induced by t-BOOH were dose-dependently prevented when cells were treated with the extracts. CB08035-SCA caused up-regulation of Nrf2, AKT1 and Bcl-2 gene expressions. Moreover, CB08035-SCA and CB08035-SYP treatments reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions of apoptosis, proinflammation and oxidative stress induced by t-BOOH. CB08035-SCA and CB08035-SYP CPE extracts confer a significant protection against oxidative insults to cells. Our results show that culture extracts CB08035-SCA and CB08035-SYP from M. hydrocarbonoclasticus (strain CB08035) appeared to have antioxidant potential, based on their ability to protect antioxidant enzymes and mRNA gene expressions linked to apoptosis/oxidative pathways. These results suggest that culture extracts CB08035-SCA and CB08035-SYP can be a potential ingredient in the pharmaceutical and cosmeceutical industries. Martínez MA, Ares I, Martínez M, Lopez-Torres B, Rodríguez JL, Maximiliano JE, Martínez-Larrañaga MR, Anadón A, Rosa JM, Cueto M. Protective effects of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) against oxidant-induced stress in human colon carcinoma Caco-2 cells. Food Chem Toxicol. 2020 Nov;145:111671. doi: 10.1016/j.fct.2020.111671. Epub 2020 Aug 9. PMID: 32784000. 0278-6915 10.1016/j.fct.2020.111671 https://hdl.handle.net/20.500.14352/97616 https://doi.org/10.1016/j.fct.2020.111671 32784000 Protective effects of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) against oxidant-induced stress in human colon carcinoma Caco-2 cells