2026-06-28T10:38:36Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/983092024-11-07T18:43:01Zcom_20.500.14352_14col_20.500.14352_15

Polymerase chain reaction (PCR) identification of Penicillium brevicompactum, a grape contaminant and mycophenolic acid producer Patiño Álvarez, Aurora Belén Medina, Ángel Domenech Lucas, Miriam González Jaén, María Teresa Jiménez, Misericordia Vázquez Estévez, María Covadonga Inmaculada Penicillium brevicompactum is a ubiquitous fungal species that contaminates diverse substrates and commodities and produces an array of metabolites toxic to human and animals. The present work has obtained evidence, by liquid chromatography (LC)-ion-trap mass spectrometry, of the ability of P. brevicompactum strains isolated from grapes to produce mycophenolic acid, a potent immunosuppressor. In order to facilitate early diagnosis of this species on commodities for human and animal consumption, a rapid, sensitive and specific polymerase chain reaction (PCR) assay for P. brevicompactum was developed. The specific primers were designed based on the ITS1-5.8S-ITS2ITS (Internal Transcribed Spacers of rRNA genes) multicopy region. This method provides a useful aid to detect the presence of this fungal species in grapes and other commodities in order to prevent the toxins produced entering the food chain. 2024-02-02T13:10:36Z 2024-02-02T13:10:36Z 2024-02-02T13:10:36Z 2007 journal article https://hdl.handle.net/20.500.14352/98309 0265-203X 10.1080/02652030600967222 1464–5122 eng (Project Nos AGL-2001-2974-C05-01 and AGL-2004-07549-C05-02/ALI) Project No. GV04B-111 Patiño, B., Medina, Á., Doménech, M., González-Jaén, M. T., Jiménez, M., & Vázquez, C. (2007). Polymerase chain reaction (PCR) identification of Penicillium brevicompactum , a grape contaminant and mycophenolic acid producer. Food Additives & Contaminants, 24(2), 165–172. https://doi.org/10.1080/02652030600967222 restricted access