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00925njm 22002777a 4500 dc Montiel, Raquel author Martín Cabrejas, Izaskun author Langa, Susana author El Aouad, Noureddine author Arqués Orobón, Juan Luis author Reyes, Fernando author Medina, Margarita author 2014 Lactobacillus reuteri INIA P579 was used for the production and purification of reuterin. The purity of reuterin was assessed by high resolution electrospray ionization mass spectrometry (HRESIMS) and nuclear magnetic resonance (NMR) spectroscopy. After purification, reuterin concentration obtained was 1.3 M. The inhibitory activity using Escherichia coli K12 as indicator strain was estimated to be 510 AU/ml. Survival curves in tryptic soy broth revealed that reuterin required to inhibit the growth of three Listeria monocytogenes strains was in the range of 2e4 AU/ml. Purified reuterin (10 AU/g) significantly reduced the growth of L. monocytogenes in cold-smoked salmon kept under moderate or strong temperature abuse conditions. After 15 d at 8 C, cold-smoked salmon with added reuterin exhibited L. monocytogenes counts 2.0 log CFU/g lower than control smoked salmon with no reuterin added. At 30 C, reuterin also controlled the growth of the pathogen, with counts 1.4 and 0.9 log CFU/g lower than those observed in control smoked salmon after 24 and 48 h, respectively. The addition of purified reuterin might be used as a hurdle technology to improve the safety and extend the shelf-life of lightly preserved seafood products such as cold-smoked salmon. Montiel, R., et al. «Antimicrobial Activity of Reuterin Produced by Lactobacillus Reuteri on Listeria Monocytogenes in Cold-Smoked Salmon». Food Microbiology, vol. 44, diciembre de 2014, pp. 1-5. https://doi.org/10.1016/j.fm.2014.05.006. 0740-0020 10.1016/j.fm.2014.05.006 https://hdl.handle.net/20.500.14352/98577 https://doi.org/10.1016/j.fm.2014.05.006 Antimicrobial activity of reuterin produced by Lactobacillus reuteri on Listeria monocytogenes in cold-smoked salmon