2026-08-24T05:19:07Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/992752024-02-07T00:50:30Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Modrego, Javier author Azcona, Luis author Martín Palacios, Naiara author Zamorano León, José Javier author Segura, Antonio author Rodriguez, Pablo author Guerra, Reddy author Tamargo Menéndez, Juan author Macaya Miguel, Carlos author López Farre, Antonio José 2024-02-06T08:52:43Z 2024-02-06T08:52:43Z 2013-10-24 Modrego J, Azcona L, Martı ́n-Palacios N, Zamorano-Leo ́n JJ, Segura A, et al. (2013) Platelet Content of Nitric Oxide Synthase 3 Phosphorylated AtSerine1177Is Associated with the Functional Response of Platelets to Aspirin. PLoS ONE 8(12): e82574. doi:10.1371/journal.pone.0082574 1932-6203 10.1371/journal.pone.008257424376548 https://hdl.handle.net/20.500.14352/99275 1932-6203 https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0082574 https://pubmed.ncbi.nlm.nih.gov/24376548/ Objective: To analyse if platelet responsiveness to aspirin (ASA) may be associated with a different ability of platelets to generate nitric oxide (NO). Patients/methods: Platelets were obtained from 50 patients with stable coronary ischemia and were divided into ASA-sensitive (n = 26) and ASA-resistant (n = 24) using a platelet functionality test (PFA-100). Results: ASA-sensitive platelets tended to release more NO (determined as nitrite + nitrate) than ASA-resistant platelets but it did not reach statistical significance. Protein expression of nitric oxide synthase 3 (NOS3) was higher in ASA-sensitive than in ASA-resistant platelets but there were no differences in the platelet expression of nitric oxide synthase 2 (NOS2) isoform. The highest NOS3 expression in ASA-sensitive platelets was independent of the presence of T-to-C mutation at nucleotide position -786 (T(-786) → C) in the NOS3-coding gene. However, platelet content of phosphorylated NOS3 at Serine (Ser)(1177), an active form of NOS3, was higher in ASA-sensitive than in ASA-resistant platelets. The level of platelet NOS3 Ser(1177) phosphorylation was positively associated with the closure time in the PFA-100 test. In vitro, collagen failed to stimulate the aggregation of ASA-sensitive platelets, determined by lumiaggregometry, and it was associated with a significant increase (p = 0.018) of NOS3 phosphorylation at Ser(1177). On the contrary, collagen stimulated the aggregation of ASA-resistant platelets but did not significantly modify the platelet content of phosphorylated NOS3 Ser(1177). During collagen stimulation the release of NO from ASA-sensitive platelets was significantly enhanced but it was not modified in ASA-resistant platelets. Conclusions: Functional platelet responsiveness to ASA was associated with the platelet content of phosphorylated NOS3 at Ser(1177). eng Attribution 4.0 International Platelet Content of Nitric Oxide Synthase 3 Phosphorylated At Serine1177 Is Associated with the Functional Response of Platelets to Aspirin journal article 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URL https://docta.ucm.es/bitstreams/91bcd186-2241-467f-920b-ad935da20aa9/download File MD5 3cbe99012116debe91b4eb3b969e80c8 506891 application/pdf Platelet Content of Nitric Oxide.pdf