2026-06-27T15:23:39Zhttps://docta.ucm.es/rest/oai/request
oai:docta.ucm.es:20.500.14352/996632024-10-10T00:06:58Zcom_20.500.14352_14col_20.500.14352_15
Docta Complutense author Sobrino Plata, Juan author Ortega Villasante, Cristina author Flores Cáceres, Laura author Escobar, Carolina author Del Campo, Francisca author Hernández, Luis 2024-02-06T17:27:01Z 2024-02-06T17:27:01Z 2009 Sobrino-Plata, Juan, et al. «Differential Alterations of Antioxidant Defenses as Bioindicators of Mercury and Cadmium Toxicity in Alfalfa». Chemosphere, vol. 77, n.o 7, noviembre de 2009, pp. 946-54. https://doi.org/10.1016/j.chemosphere.2009.08.007. 0045-6535 10.1016/j.chemosphere.2009.08.007 https://hdl.handle.net/20.500.14352/99663 https://doi.org/10.1016/j.chemosphere.2009.08.007 https://pubmed.ncbi.nlm.nih.gov/19732935/ Several physiological parameters related to oxidative stress, which is a characteristic of plants exposed to toxic metals, were studied in 3-week-old alfalfa plants treated with cadmium (Cd) or mercury (Hg) at doses of 0, 3, 10 and 30 μM for 7 d. The concentrations of biothiols, glutathione (GSH), homoglutathione (hGSH) and phytochelatins (PCs) increased dramatically in metals-treated plants, in particular in the presence of Cd. This was accompanied by a remarkable up-regulation of γ-glutamyl cysteine synthetase gene, probably in response to the higher demand for GSH|hGSH needed for PC synthesis. The presence of metals enhanced lipid peroxidation in shoots, while chlorophyll content declined in a concentration dependent manner. Ascorbate peroxidase (APX) activity increased moderately in roots of Cd-exposed plants, and a new basic root peroxidase isoform was found in both Cd- and Hg-treated plants. Glutathione reductase (GR) activity was enhanced in shoots of plants exposed to Cd and Hg. However, this enzymatic activity showed a metal dependent response in roots, and was enhanced in Cd-treated plants but was severely inhibited in roots of plants treated with Hg. Inhibition of GR by Hg was confirmed in vitro by incubating a commercially available GR and control shoot extracts with several doses of Hg and Cd. Ascorbate concentrations were elevated with treatments of 3 μM Hg, 10 μM Cd and 30 μM Cd, indicating that this compound is necessary for redox cellular homeostasis. The different responses observed with Cd and Hg treatments might be the basis for specific stress bioindicators. eng Differential alterations of antioxidant defenses as bioindicators of mercury and cadmium toxicity in alfalfa journal article 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URL https://docta.ucm.es/bitstreams/5575ccde-1b64-48bf-a718-1ca46fc714fa/download File MD5 f49d674a73cb209e35ccfd44b8f34aad 766090 application/pdf Differential_alterations.pdf