2026-06-29T02:52:44Zhttps://docta.ucm.es/rest/oai/request

oai:docta.ucm.es:20.500.14352/999762024-05-08T12:26:24Zcom_20.500.14352_14col_20.500.14352_15

Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications Amorim, Adriany das Graças Nascimento Sánchez-Paniagua López, Marta Oliveira, Taiane Maria de Mafud, Ana Carolina Alves da Silva, Durcilene Souza de Almeida Leite, José Roberto de López Ruiz, María Beatriz This research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between –NH and –OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 53 nm and a polydispersity index of 0.309 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 mA mM1 cm2 ), wide linear range of 2.5 107 –2.0 104 M, and low detection limit (50 nM). 2024-02-07T12:55:19Z 2024-02-07T12:55:19Z 2024-02-07T12:55:19Z 2021-08-02 journal article https://hdl.handle.net/20.500.14352/99976 2050-750X 10.1039/d1tb01164b 2050-7518 eng Das Graças Nascimento Amorim A, Sánchez-Paniagua M, De Oliveira TM, Mafud AC, Da Silva DA, De Souza De Almeida Leite JR, et al. Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications. J Mater Chem B 2021;9:6825–35. https://doi.org/10.1039/D1TB01164B. restricted access