Muñoz Maceda, AnaPriego González, AndreaNúñez Puente, CarolinaRizos, DimitriosCerdeira Lozano, Joaquín VicenteRoldán, EduardoSánchez Calabuig, María JesúsSánchez Rodríguez, Ana2024-10-302024-10-302024Munoz-Maceda, A., Priego-Gonzalez, A., Núñez-Puente, C., Rizos, D., Cerdeira-Lozano, J., Sanchez-Rodriguez, A., Roldan, E. R. S., & Sánchez-Calabuig, M. J. (2024). Enhancing felid conservation: Exploring the impact of in vitro culture media on domestic cat blastocyst production. Reproduction in Domestic Animals, 59(Suppl. 3), e14645. https://doi.org/10.1111/rda.146450936-676810.1111/rda.14645https://hdl.handle.net/20.500.14352/109804This study investigated the optimization of assisted reproductive techniques for wild felid conservation, focusing on in vitro procedures using the domestic cat as a model species. The research evaluated the impact of three different in vitro culture media on blastocyst formation. Oocytes and spermatozoa were collected and processed, followed by in vitro fertilization and culture. Results returned a similar blastocyst rate (ANOVA, p > .05), over 16% across all groups. While demonstrating the potential of these techniques, further investigations are warranted to evaluate embryo quality to refine optimal protocols and their applicability in felid conservation efforts.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1439-0531https://doi.org/10.1111/rda.1464539396873open access636.09Blastocyst formationFeline reproductionIn vitro cultureVeterinaria3109 Ciencias Veterinarias