Calderón, JosselaMaganto-Garcia, ElenaPunzón, CarmenTerhorst, CoxFresno, ManuelCarrión Herrero, Francisco JavierSacks, David L.2024-01-182024-01-182012-07-12Calderón J, Maganto-Garcia E, Punzón C, Carrión J, Terhorst C, Fresno M (2012) The Receptor Slamf1 on the Surface of Myeloid Lineage Cells Controls Susceptibility to Infection by Trypanosoma cruzi. PLoS Pathog 8(7): e1002799. https://doi.org/10.1371/journal.ppat.10027991553-737410.1371/journal.ppat.1002799https://hdl.handle.net/20.500.14352/93867Author Contributions Conceived and designed the experiments: J. Calderon, E. Maganto-Garcia, M. Fresno. Performed the experiments: J. Calderon, E. Maganto-Garcia, C. Punzon, J. Carrion. Analyzed the data: J. Calderon, E. Maganto-Garcia, C. Terhorst, M. Fresno. Contributed reagents/materials/analysis tools: C. Terhorst. Wrote the paper: C. Terhorst, M. Fresno.Trypanosoma cruzi, the protozoan parasite responsible for Chagas' disease, causes severe myocarditis often resulting in death. Here, we report that Slamf1-/- mice, which lack the hematopoietic cell surface receptor Slamf1, are completely protected from an acute lethal parasite challenge. Cardiac damage was reduced in Slamf1-/- mice compared to wild type mice, infected with the same doses of parasites, as a result of a decrease of the number of parasites in the heart even the parasitemia was only marginally less. Both in vivo and in vitro experiments reveal that Slamf1-defIcient myeloid cells are impaired in their ability to replicate the parasite and show altered production of cytokines. Importantly, IFN-γ production in the heart of Slamf1 deficient mice was much lower than in the heart of wt mice even though the number of infiltrating dendritic cells, macrophages, CD4 and CD8 T lymphocytes were comparable. Administration of an anti-Slamf1 monoclonal antibody also reduced the number of parasites and IFN-γ in the heart. These observations not only explain the reduced susceptibility to in vivo infection by the parasite, but they also suggest human Slamf1 as a potential target for therapeutic target against T. cruzi infection.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://journals.plos.org/plospathogens/article?id=10.1371/journal.ppat.100279922807679open access636.09AnimalsAntibodies, MonoclonalAntibodies, ProtozoanAntigens, CDCD4-Positive T-LymphocytesCD8-Positive T-LymphocytesCells, CulturedChagas CardiomyopathyChagas DiseaseCytokinesDendritic CellsDisease SusceptibilityHeartInterferon-gammaMacrophagesMiceMice, Inbred BALB CMice, KnockoutMyeloid CellsMyocardiumParasitemiaReceptors, Cell SurfaceTrypanosoma cruziVeterinaria24 Ciencias de la Vida