García Herranz, DavidBravo Osuna, IreneGarcía Feijoo, JuliánHerrero Vanrell, María Del RocíoRodrigo, María JesúsSubías, ManuelMontolío, AlbertoMéndez-Martínez, SilviaMartínez-Rincón, TeresaArias, LorenaPablo, Luis ECegoñino, JoséCarretero, AnaRuberte, JesúsGarcía-Martín, ElenaPérez del Palomar, Amaya2025-02-112025-02-112021Rodrigo, M.J.; Subías, M.; Montolío, A.; Méndez-Martínez, S.; Martínez-Rincón, T.; Arias, L.; García-Herranz, D.; Bravo-Osuna, I.; Garcia-Feijoo, J.; Pablo, L.; et al. Analysis of Parainflammation in Chronic Glaucoma Using Vitreous-OCT Imaging. Biomedicines 2021, 9, 1792. https://doi.org/ 10.3390/biomedicines912179210.3390/biomedicines9121792https://hdl.handle.net/20.500.14352/117981Glaucoma causes blindness due to the progressive death of retinal ganglion cells. The immune response chronically and subclinically mediates a homeostatic role. In current clinical practice, it is impossible to analyse neuroinflammation non-invasively. However, analysis of vitreous images using optical coherence tomography detects the immune response as hyperreflective opacities. This study monitors vitreous parainflammation in two animal models of glaucoma, comparing both healthy controls and sexes over six months. Computational analysis characterizes in vivo the hyperreflective opacities, identified histologically as hyalocyte-like Iba-1+ (microglial marker) cells. Glaucomatous eyes showed greater intensity and number of vitreous opacities as well as dynamic fluctuations in the percentage of activated cells (50–250 microns2) vs. non-activated cells (10–50 microns2), isolated cells (10 microns2) and complexes (>250 microns2). Smaller opacities (isolated cells) showed the highest mean intensity (intracellular machinery), were the most rounded at earlier stages (recruitment) and showed the greatest change in orientation (motility). Study of vitreous parainflammation could be a biomarker of glaucoma onset and progression.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3390/biomedicines9121792open access615.4617.7Optical coherence tomographyBiomedical imagingImage analysis and processingVitreous bodyGlaucomaAnimal modelsInflammationOftalmologíaTecnología farmaceútica3201.09 Oftalmología