Del Río, ALópez-Gordillo, YMartínez, M LBarrio Asensio, María Del CarmenMurillo Arroyo, Francisco JavierMaldonado Bautista, EstelaMartínez Sanz, ElenaMartínez Álvarez, María Concepción2024-02-142024-02-142011Del Rio, A., Barrio, M. C., Murillo, J., Maldonado, E., Lopez-Gordillo, Y., Martínez-Sanz, E., ... & Martinez-Alvarez, C. (2011). Analysis of the presence of cell proliferation-related molecules in the Tgf-β3 null mutant mouse palate reveals misexpression of EGF and Msx-1. Cells Tissues Organs, 193(3), 135-150.1422-640510.1159/000319970https://hdl.handle.net/20.500.14352/101443The Tgf-β3 null mutant mouse palate presents several cellular anomalies that lead to the appearance of cleft palate. One of them concerns the cell proliferation of both the palatal medial edge epithelium and mesenchyme. In this work, our aim was to determine whether there was any variation in the presence/distribution of several cell proliferation-related molecules that could be responsible for the cell proliferation defects observed in these palates. Our results showed no difference in the presence of EGF-R, PDGF-A, TGF-β2, Bmp-2, and Bmp-4, and differences were minimal for FGF-10 and Shh. However, the expression of EGF and Msx-1 changed substantially. The shift of the EGF protein expression was the one that most correlated with that of cell proliferation. This molecule is regulated by TGF-β3, and experiments blocking its activity in culture suggest that EGF misexpression in the Tgf-β3 null mutant mouse palate plays a role in the cell proliferation defect observed.engAttribution-NonCommercial-NoDerivatives 4.0 Internationaljournal articlehttps://karger.com/cto/article/193/3/135/91146/Analysis-of-the-Presence-of-Cell-Proliferationopen access611.013611Tgf beta3 KnockoutPalateEpidermal growth factorMsx-1Ciencias Biomédicas32 Ciencias Médicas24 Ciencias de la Vida