Daza, María A.Miró Corrales, GuadalupeFragio Arnold, CristinaPérez Montero, BlancaBelinchón Esteban, SheilaFermín Rodríguez, María Luisa2024-08-272024-08-272024-08-01Daza, M. A., Miró, G., Fragío, C., Perez-Montero, B., Esteban, S. B., & Fermín, M. L. (2024). Urinary Tamm-Horsfall protein excretion is a potential early biomarker of renal distal tubular damage in canine leishmaniosis. Veterinary parasitology, 331, 110265. Advance online publication. https://doi.org/10.1016/j.vetpar.2024.11026510.1016/j.vetpar.2024.110265https://hdl.handle.net/20.500.14352/107696This study evaluates distal tubular damage in early stages of renal disease in dogs with naturally acquired leishmaniosis. Pherograms of urinary proteins separated in vertical electrophoresis system (SDS-PAGE) were evaluated. Peptide fingerprint and fragmentation (MALDI-TOF TOF) identified bands located at 100 and 60 kDa as Tamm-Horsfall protein (THP) and albumin, respectively. The variables examined were: urine protein to creatinine ratio (UPC), total number of bands, quantification of THP urinary excretion through the optical density (OD %) of bands located at 100 kDa, blood creatinine, and urine specific gravity (USG). Positive correlation was found between UPC and the number of bands (ρ = 0.75849, P = <0.0001). Negative correlation was identified between UPC and OD % of 100 kDa bands (ρ = −0.85332, P = <0.0001), and the number of bands and OD % of 100 kDa bands (ρ = −0.74479, P = <0.0001). The area under the ROC curve was 0.991 (95 % CI, 0.976–1). The optimal cut-off UPC that better discriminated between urines with high or low OD% of THP was 0.46 with 92.6 % sensitivity and 96.2 % specificity. Our findings indicate that non azotemic dogs with borderline proteinuria might excrete low amount of THP, which could suggest tubular damage in early stages of chronic kidney disease.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1873-2550https://doi.org/10.1016/j.vetpar.2024.11026539094329restricted access636.7.09:616Canine leishmaniosisRenal functionUrinary Tamm-Horsfall proteinDogTubular damageVeterinaria3109 Ciencias Veterinarias