Martínez Caballero, María AranzazuAres Lombán, IrmaMartínez Caballero, MartaLópez Torres, BernardoMaximiliano Guerra, Jorge EnriqueRodriguez, José LuisMartínez Larrañaga, María RosaAnadón Navarro, Arturo RamónPeteiro, CesarRubiño, SusanaHortos, María2024-02-012024-02-012021Martínez MA, Ares I, Martínez M, Lopez-Torres B, Maximiliano JE, Rodríguez JL, Martínez-Larrañaga MR, Anadón A, Peteiro C, Rubiño S, Hortos M. Brown marine algae Gongolaria baccata extract protects Caco-2 cells from oxidative stress induced by tert-butyl hydroperoxide. Food Chem Toxicol. 2021 Oct;156:112460. doi: 10.1016/j.fct.2021.112460. Epub 2021 Aug 1. PMID: 34348126.0278-691510.1016/j.fct.2021.112460https://hdl.handle.net/20.500.14352/97677Gongolaria baccata (S.G. Gmelin) is marine brown seaweed mainly found on the coasts of the Baltic Sea south to the Mediterranean Sea, Canary Islands, Mauritania and Western Sahara. Herein, we report the cell viability and protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butylhydroperoxide (tert-BOOH). The extract prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with tert-BOOH. The increases of MDA levels, the amount of intracellular ROS and caspase 3/7 activity induced by tert-BOOH were prevented when cells were treated with the G. baccata extract. Moreover, G. baccata extract caused up-regulation of GSTM2, Nrf2, and AKT1 gene expressions, as well as G. baccata extract reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, P38, P53, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions related to apoptosis, proinflammation and oxidative stress induced by tert-BOOH. These results suggest that G.baccata extract protected the cells against oxidative damage and inflammation; protective effects that could be linked to their bioactive constituents. Hence, this brown seaweed G. baccata extract could be used for the development of functional foods and/or nutraceuticals.engjournal articlehttps://doi.org/10.1016/j.fct.2021.11246034348126resrticted access615.9G. baccata extractPhenolic and carbohydrate derivativesOxidative stressCaco-2 cellsCiencias Biomédicas3214 Toxicología