Villegas, María FernandaGarcía Uriostegui, LorenaRodríguez, OfeliaIzquierdo Barba, IsabelSalinas Sánchez, Antonio JesúsToriz, GuillermoVallet Regí, María Dulce NombreDelgado, Ezequiel2023-06-172023-06-172017Villegas, M. F., García Uriostegui, L., Rodríguez, O. et al. «Lysine-Grafted MCM-41 Silica as an Antibacterial Biomaterial». Bioengineering, vol. 4, n.o 4, septiembre de 2017, p. 80. DOI.org (Crossref), https://doi.org/10.3390/bioengineering4040080.2306-5354; CODEN: BIOEBG10.3390/bioengineering4040080https://hdl.handle.net/20.500.14352/18120ORCID 0000-0002-6104-4889 (María Vallet Regí) RESEARCHER ID M-3378-2014 (María Vallet Regí)Abstract: A facile strategy for zwitterionization of bioceramics based on direct incorporation of L-lysine amino acid via the ε-amino group onto mesoporous MCM-41 materials is proposed. FTIR studies of lysine-grafted MCM-41 (MCM-LYS) showed simultaneously bands at 3080 and 1540 cm−1 and bands at 1625 and 1415 cm−1 corresponding to -NH3+/COO− pairs, demonstrating the incorporation of the amino acid on the material surface keeping its zwitterionic character. Both elemental and thermogravimetric analyses showed that the amount of grafted lysine was 8 wt % based on the bioceramic total weight. Moreover, MCM-LYS material exhibited a reduction of adhesion of S. aureus and E. coli bacteria in 33 and 50%, respectively at physiological pH, as compared with pristine MCM-41. Biofilm studies onto surfaces showed that lysine functionalization elicited a reduction of the area covered by S. aureus biofilm from 42% to only 5% (88%). This research shows a simple and effective approach to chemically modify bioceramics using single amino acids that provide zwitterionic functionality, useful to develop new biomaterials able to resist bacterial adhesion.engAttribution 4.0 Internationalhttps://creativecommons.org/licenses/by/4.0/journal articlehttps//doi.org/10.3390/bioengineering4040080http://www.mdpi.com/journal/bioengineeringopen access546615.46Lysine graftingZwitterionizationMesoporous MCM-41 biomaterialAntibacterial adhesionBiofilm formationIngeniería químicaQuímica inorgánica (Química)3303 Ingeniería y Tecnología Químicas2303 Química Inorgánica