Fernandez Novo, AitorSantos López, SergioBarrajon Masa, ClaraMozas, Patriciade Mercado, EduardoCaceres, ElisaGarrafa, AizicGonzález Martín, Juan VicentePerez Villalobos, NatividadOliet, AgustinAstiz, SusanaPerez Garnelo, Sonia Salome2025-01-232025-01-232021Fernandez-Novo, A., Santos-Lopez, S., Barrajon-Masa, C., Mozas, P., de Mercado, E., Caceres, E., Garrafa, A., Gonzalez-Martin, J. V., Perez-Villalobos, N., Oliet, A., Astiz, S., & Perez-Garnelo, S. S. (2021). Effects of Extender Type, Storage Time, and Temperature on Bull Semen Parameters. Biology, 10(7), 630. https://doi.org/10.3390/biology1007063010.3390/biology10070630https://hdl.handle.net/20.500.14352/115905Author Contributions Conceptualization: A.F.-N., S.S.-L., C.B.-M., J.V.G.-M., N.P.-V., A.O., S.A. and S.S.P.-G.; methodology, A.F.-N., S.S.-L., C.B.-M., P.M., E.d.M., E.C., A.G., S.A. and S.S.P.-G.; software, A.F.-N., C.B.-M., P.M., E.d.M., E.C., A.G., A.O. and S.A.; validation, J.V.G.-M., N.P.-V., A.O., S.A. and S.S.P.-G. ; formal analysis, A.F.-N., S.A. and S.S.P.-G.; investigation, A.F.-N., P.M., E.d.M., E.C., A.G., A.O., S.A. and S.S.P.-G.; resources, A.F.-N., S.S.-L., N.P.-V., A.O., S.A. and S.S.P.-G.; data curation, A.F.-N., S.A. and S.S.P.-G.; writing—original draft preparation, A.F.-N., S.A. and S.S.P.-G.; writing—review and editing, A.F.-N., J.V.G.-M., N.P.-V., S.A. and S.S.P.-G.; visualization, S.A. and S.S.P.-G.; supervision, S.S.-L., J.V.G.-M., N.P.-V., S.A. and S.S.P.-G.; project administration, S.A. and S.S.P.-G.; funding acquisition, S.A. and S.S.P.-G. All authors have read and agreed to the published version of the manuscript.Seminal parameters can be evaluated in situ, or samples can be delivered to a diagnostic centre. How storage conditions affect ejaculates up to evaluation is unclear. We assessed, in 25 commercial bulls electroejaculated in the field, the impact of time until evaluation (0–2 h, 4–6 h, and 24 h post-ejaculation), holding temperature (5 °C vs. room temperature), and extender (AndroMed®, BIOXcell® or INRA96®) on semen quality. Acrosome integrity, sperm viability and morphology, CASA-total and progressive motility, pH, and colony-forming units were assessed. Semen quality was preserved for up to 4–6 h post-ejaculation, except for INRA96® at 5 °C. Regardless of extender or temperature, motility decreased from 4 to 6 h up to 24 h, with the best values obtained with BIOXcell® at 5 °C. pH differed from 4 to 6 h up to 24 h, acidifying when stored at room temperature. Microbiological load was stable over time with AndroMed® and BIOXcell®, and increased at room temperature with INRA96®. Our results suggest that AndroMed® and BIOXcell® can preserve semen quality for up to 6 h, either at 5 °C or room temperature, while INRA96® only at room temperature. These results help to fix adequate protocols for short-term storage and shipment of bovine semen collected under field conditions.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article2079-7737https://doi.org/10.3390/biology1007063034356485https://www.mdpi.com/journal/biologyopen access611.019636.09:611.013BBSEShort-term storageSemen extenderSperm viabilitySperm motilityMicrobiological loadAnatomía veterinariaObstetricia veterinaria3104 Producción Animal2401.07 Embriología Animal