Cea Callejo, PabloArca Lafuente, SoniaGómez-Lucía Duato, María EsperanzaDomenech Gómez, Ana MaríaBiarnés, MarBlanco, ÁngelaBenítez Rico, LauraMadrid González, Ricardo2024-08-022024-08-022024-07-10Cea-Callejo, P., Arca-Lafuente, S., Gomez-Lucia, E. et al. An affordable detection system based on RT-LAMP and DNA-nanoprobes for avian metapneumovirus. Appl Microbiol Biotechnol 108, 414 (2024). https://doi.org/10.1007/s00253-024-13243-x0175-759810.1007/s00253-024-13243-xhttps://hdl.handle.net/20.500.14352/107361Airborne animal viral pathogens can rapidly spread and become a global threat, resulting in substantial socioeconomic and health consequences. To prevent and control potential epidemic outbreaks, accurate, fast, and affordable point-of-care (POC) tests are essential. As a proof-of-concept, we have developed a molecular system based on the loop-mediated isothermal amplification (LAMP) technique for avian metapneumovirus (aMPV) detection, an airborne communicable agent mainly infecting turkeys and chickens. For this purpose, a colorimetric system was obtained by coupling the LAMP technique with specific DNA-functionalized AuNPs (gold nanoparticles). The system was validated using 50 different samples (pharyngeal swabs and tracheal tissue) collected from aMPV-infected and non-infected chickens and turkeys. Viral detection can be achieved in about 60 min with the naked eye, with 100% specificity and 87.88% sensitivity for aMPV. In summary, this novel molecular detection system allows suitable virus testing in the field, with accuracy and limit of detection (LOD) values highly close to qRT-PCR-based diagnosis. Furthermore, this system can be easily scalable to a platform for the detection of other viruses, addressing the current gap in the availability of POC tests for viral detection in poultry farming.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1432-0614https://doi.org/10.1007/s00253-024-13243-x38985204open access636.09Molecular detectionReverse transcription loop mediated isothermal amplifcation (RT-LAMP)NanoprobesAvian metapneumovirus (aMPV)Point of Care (POC) testVeterinaria3109 Ciencias Veterinarias