Acosta, JavierDel Arco, JonMartínez Pascual, SaraClemente Suárez, Vicente JavierFernández Lucas, Jesús2024-11-082024-11-082018Acosta, J., Del Arco, J., Martinez-Pascual, S., Clemente-Suárez, V. J., & Fernández-Lucas, J. (2018). One-pot multi-enzymatic production of purine derivatives with application in pharmaceutical and food industry. Catalysts, 8(1). https://doi.org/10.3390/CATAL80100092073-434410.3390/catal8010009https://hdl.handle.net/20.500.14352/110367Biocatalysis reproduce nature’s synthetic strategies in order to synthesize different organic compounds. Natural metabolic pathways usually involve complex networks to support cellular growth and survival. In this regard, multi-enzymatic systems are valuable tools for the production of a wide variety of organic compounds. Methods: The production of different purine nucleosides and nucleoside-5′-monophosphates has been performed for first time, catalyzed by the sequential action of 2′-deoxyribosyltransferase from Lactobacillus delbrueckii (LdNDT) and hypoxanthine-guanine-xanthine phosphoribosyltransferase from Thermus themophilus HB8 (TtHGXPRT). Results: The biochemical characterization of LdNDT reveals that the enzyme is active and stable in a broad range of pH, temperature, and ionic strength. Substrate specificity studies showed a high promiscuity in the recognition of purine analogues. Finally, the enzymatic production of different purine derivatives was performed to evaluate the efficiency of multi-enzymatic system LdNDT/TtHGXPRT. Conclusions: The production of different therapeutic purine nucleosides was efficiently catalyzed by LdNDT/TtHGXPRT. In addition, the resulting by-products were converted to IMP and GMP. Taking all of these features, this bioprocess entails an efficient, sustainable, and economical alternative to chemical synthetic methods.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3390/catal8010009https://www.mdpi.com/2073-4344/8/1/9open access577.15577.123615.316642′-deoxyribosyltransferasePhosphoribosyltransferasesCascade reactionsPurine nucleoside analoguesDietary nucleotidesBioquímica (Biología)BiotecnologíaQuímica industrialQuímica farmaceútica2403 Bioquímica2302.09 Enzimología2302.23 Ácidos Nucleicos2390 Química Farmacéutica2302.90 Bioquímica de Alimentos