Gosálvez Berenguer, JaimeRamírez, Miguel ÁngelLópez Fernández, CarmenCrespo Castejón, FranciscoEvans, MichaelKjelland, MichaelMoreno, Juan Francisco2024-01-312024-01-312011Gosálvez J, Ramirez MA, López-Fernández C, Crespo F, Evans KM, Kjelland ME, Moreno JF. Sex-sorted bovine spermatozoa and DNA damage: I. Static features. Theriogenology. 2011 Jan 15;75(2):197-2050093-691X10.1016/j.theriogenology.2010.08.006https://hdl.handle.net/20.500.14352/97424This study examined the static response of Spermatozoa DNA Fragmentation (SDF) after sex selection in bulls using a MoFlo® SX (Beckman Coulter, Miami FL) spermatozoa sorter to produce three different subpopulations: 1) Spermatozoa bearing X- chromosomes with a purity of 95%, 2) Spermatozoa bearing Y-chromosomes with a purity of 95%, and 3) non-viable spermatozoa. The static response of SDF refers to the baseline values observed for DNA damage when analyzed pre- and post sex-sorting. Results showed that while the baseline level SDF in pre-sorted bull spermatozoa samples ranged from 5.3% to 11% with an average of 7.9% ± 2.1%, the level of SDF obtained in X- and Y-chromosome sorted samples was much lower (3.1% ± 1.9%) and statistical differences were obtained after comparing both groups (P < 0.01). Spermatozoa containing a fragmented DNA molecule tend to be accumulated in the non-viable subpopulation. The baseline SDF level in X- and Y-chromosome sorted subpopulations is reduced, by 63% on average when compared to the values obtained in the neat semen sample. Different bulls exhibit unique SDF reduction efficiencies via the X- and Y-chromosome sex selection process.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.theriogenology.2010.08.00620932559https://pubmed.ncbi.nlm.nih.gov/20932559/restricted access61Bovine reproductionAndrologyFlow cytometrySex-sortingSperm DNA fragmentationVeterinaria24 Ciencias de la Vida