A mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway disease

Angelina Querencias, AlbaLavín Plaza, BegoñaMartín De La Cruz, LeticiaPalomares Gracia, ÓscarPérez Diego, MarioConejero, LauraGayá, AubaMaldonado, ÁngelMarcos-Ramiro, BeatrizPérez de Llano, LuisReal, Francisco XavierSevilla Ortega, CarmenSubiza, José Luis2026-02-242026-02-242025-08-03Sevilla-Ortega C, Angelina A, Martín-Cruz L, Pérez-Diego M, Maldonado A, Lavín B, Marcos-Ramiro B, Pérez de Llano L, Gayá A, Real FX, Conejero L, Subiza JL, Palomares O. A mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway disease. Nat Commun. 2025 Aug 3;16(1):7129. doi: 10.1038/s41467-025-62632-x10.1038/s41467-025-62632-xhttps://hdl.handle.net/20.500.14352/132959Allergic sensitization and viral infections are risk factors for asthma development and progression. Sublingual vaccination with MV130, a whole heat-inactivated polybacterial preparation, protects against viral infections, but its impact on allergic sensitization and asthma development remains unknown. Here we show MV130 prevents house dust mite (HDM)-induced local type 2 immune responses and associated eosinophilic airway inflammation, conferring protection up to 9 weeks after vaccination. MV130 reduces pathophysiological and clinical asthma features in an in vivo experimental mouse model of HDM-induced allergic eosinophilic asthma, restoring normal airway functionality. MV130 impairs allergen-specific IgE sensitization and systemic type 2 inflammation endorsing type 1 and IL-10 responses. In human DCs, MV130 induces a transcriptomic and metabolic reprogramming, and restores non-pathological immune responses to allergens in healthy and asthmatic donors. Additionally, the adoptive transfer of MV130-stimulated BMDCs was sufficient to reproduce the protective features of the vaccine administration in vivo. Collectively, we show MV130 reduces allergic sensitization and eosinophilic asthma. Our findings support the exploration of mucosal interventions aimed at reducing the risk of allergen-induced asthma development.engA mucosal vaccine prevents eosinophilic allergic airway inflammation by modulating immune responses to allergens in a murine model of airway diseasejournal articlehttps://doi.org/10.1038/s41467-025-62632-xhttps://www.nature.com/articles/s41467-025-62632-xopen access577Bioquímica (Biología)Inmunología2403 Bioquímica2412 Inmunología