Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activity

Orea-Soufi, AlbaDávila, DavidSalazar Roa, MaríaLorente Pérez, María Del MarVelasco Díez, GuillermoLink, Wolfgang2023-06-172023-06-172019978-1-4939-8900-310.1007/978-1-4939-8900-3https://hdl.handle.net/20.500.14352/14005Phosphorylation of FOXO transcription factors is one of the key mechanisms involved in the regulation of the activity, nucleo-cytosolic shuttling, and stability of this family of proteins. Here, we describe several experimental approaches allowing analysis of changes in the phosphorylation of these proteins upon exposure to different stimuli.engPhosphorylation of FOXO Proteins as a Key Mechanism to Regulate Their Activitybook parthttps://link.springer.com/protocol/10.1007%2F978-1-4939-8900-3_5restricted access577.1577.2FOXO phosphorylationWestern blotImmunofluorescencecell fractioningBiología molecular (Biología)Bioquímica (Biología)2415 Biología Molecular2302 Bioquímica