Gómez Mejía, EstherVicente Zurdo, DavidRosales Conrado, NoeliaLeón González, María Eugenia DeMadrid Albarrán, María Yolanda2023-06-222023-06-222022-09-160023-643810.1016/j.lwt.2022.113988https://hdl.handle.net/20.500.14352/72110CRUE-CSIC (Acuerdos Transformativos 2022)The integrated valorisation of waste from the food chain to obtain value-added compounds with biological functionality will facilitate the transition to the era of a sustainable bioeconomy. To this end, an efficient matrix solid-phase dispersion (MSPD) extraction method was developed and optimized, using experimental factorial design and response surface methodology, for polyphenols recovery from pressed grape seeds obtained after the extraction of essential oils by cold pressing. Gallic, dihydroxybenzoic, p-coumaric and trans-ferulic acid, naringin, resveratrol, quercetin and kaempferol were quantified at 2.1–295 μg g−1 by capillary liquid chromatography coupled to a diode array detector and a mass analyser (cLC-DAD-MS). Furthermore, total antioxidant activity, free radical scavenging and lipid peroxidation suppression, together with the inhibition of beta-amyloid (Αβ42) protein aggregation, considered one of the main pathological effects of Alzheimer's disease, were evaluated. Potent lipid peroxidation inhibition (IC50 0.238 ± 0.003 ng g−1) was observed, along with the reduction of Αβ42 fibril width (9.4–54.8%) and aggregation. The results presented proved that the MSPD extraction method could be considered as an efficient and sustainable methodology to produce phenolic-rich extracts that may serve as an alternative antioxidant and neuroprotective ingredient for the food or pharmaceutical formulations, leading to the cascade valorisation of winery by-products.engAtribución-NoComercial-SinDerivadas 3.0 Españajournal articlehttps://doi.org/10.1016/j.lwt.2022.113988open access543PolyphenolsPressed grape seedsMatrix solid-phase dispersionAntioxidant propertiesΑβ42 aggregation inhibitionQuímica analítica (Química)2301 Química Analítica