Martínez Relimpio, Ana MaríaBenito, MartaPérez Izquierdo, ElenaTeijón López, CésarOlmo López, Rosa MaríaBlanco Gaitán, María Dolores2026-01-152026-01-152021-06-24Martínez-Relimpio AM, Benito M, Pérez-Izquierdo E, Teijón C, Olmo RM, Blanco MD. Paclitaxel-loaded folate-targeted albumin-alginate nanoparticles crosslinked with ethylenediamine. Synthesis and in vitro characterization. Polymers. 2021;13(13).10.3390/polym13132083https://hdl.handle.net/20.500.14352/130383Among the different ways to reduce the secondary effects of antineoplastic drugs in cancer treatment, the use of nanoparticles has demonstrated good results due to the protection of the drug and the possibility of releasing compounds to a specific therapeutic target. The α-isoform of the folate receptor (FR) is overexpressed on a significant number of human cancers; therefore, folate-targeted crosslinked nanoparticles based on BSA and alginate mixtures and loaded with paclitaxel (PTX) have been prepared to maximize the proven antineoplastic activity of the drug against solid tumors. Nanometric-range-sized particles (169 ± 28 nm–296 ± 57 nm), with negative Z-potential values (between −0.12 ± 0.04 and −94.1± 0.4), were synthesized, and the loaded PTX (2.63 ± 0.19–3.56 ±0.13 µg PTX/mg Np) was sustainably released for 23 and 27 h. Three cell lines (MCF-7, MDA-MB-231 and HeLa) were selected to test the efficacy of the folate-targeted PTX-loaded BSA/ALG nanocarriers. The presence of FR on the cell membrane led to a significantly larger uptake of BSA/ALG–Fol nanoparticles compared with the equivalent nanoparticles without folic acid on their surface. The cell viability results demonstrated a cytocompatibility of unloaded nanoparticle– Fol and a gradual decrease in cell viability after treatment with PTX-loaded nanoparticle–Fol due to the sustainable PTX release.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article2073-4360https://doi.org/10.3390/POLYM13132083https://www.mdpi.com/2073-4360/13/13/2083open access61Folate-targeted nanoparticlesBSA/alginate nanocarriersPaclitaxelCellular uptakeCell viabilityCiencias Biomédicas3299 Otras Especialidades Médicas