Ramírez Sebastián, Ana IsabelHoz Montañana, María Rosa DeFernández Albarral, JoséGarcía Martín, Elena SalobrarRojas López, María BlancaValiente Soriano, Francisco J.Avilés Trigueros, MarcelinoVillegas Pérez, María P.Vidal Sanz, ManuelTriviño Casado, AlbertoRamírez Sebastián, José ManuelSalazar Corral, Juan José2024-05-062024-05-062020-03-17Ramírez, A.I., de Hoz, R., Fernández-Albarral, J.A. et al. Time course of bilateral microglial activation in a mouse model of laser-induced glaucoma. Sci Rep 10, 4890 (2020). https://doi.org/10.1038/s41598-020-61848-92045-232210.1038/s41598-020-61848-9https://hdl.handle.net/20.500.14352/103770Received: 24 October 2018; Accepted: 03 March 2020; Published: 17 March 2020Microglial activation is associated with glaucoma. In the model of unilateral laser-induced ocular hypertension (OHT), the time point at which the inflammatory process peaks remains unknown. Different time points (1, 3, 5, 8, and 15 d) were compared to analyze signs of microglial activation both in OHT and contralateral eyes. In both eyes, microglial activation was detected in all retinal layers at all time points analyzed, including: i) increase in the cell number in the outer segment photoreceptor layer and plexiform layers (only in OHT eyes) from 3 d onward; ii) increase in soma size from 1 d onward; iii) retraction of the processes from 1 d in OHT eyes and 3 d in contralateral eyes; iv) increase in the area of the retina occupied by Iba-1+ cells in the nerve fiber layer/ganglion cell layer from 1 d onward; v) increase in the number of vertical processes from 1 d in contralateral eyes and 3 d in OHT eyes. In OHT eyes at 24 h and 15 d, most Iba-1+ cells were P2RY12+ and were down-regulated at 3 and 5 d. In both eyes, microglial activation was stronger at 3 and 5 d (inflammation peaked in this model). These time points could be useful to identify factors implicated in the inflammatory process.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.1038/s41598-020-61848-9https://www.nature.com/articles/s41598-020-61848-9open access617.735617.7-007.681Animal disease modelsMicrogliaRetinaOftalmología3201.09 Oftalmología